Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells

In the study presented here, a novel chlorobenzylated bi-heterocyclic hybrid molecule (7) was synthesized and its structural confirmation was carried out by IR, 1H-NMR, 13C-NMR and CHN analysis data. This compound 7 was subjected to biological study with B16F10 mouse melanoma cells. The anti-prolife...

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Main Authors:	Muhammad Athar Abbasi, Seon-Mi Yu, Aziz-ur-Rehman, Sabahat Zahra Siddiqui, Song Ja Kim, Hussain Raza, Mubashir Hassan, Abdul Rehman Sadiq Butt, Syed Adnan Ali Shah, Sung-Yum Seo
Format:	Article
Language:	English
Published:	Elsevier 2019-01-01
Series:	Toxicology Reports
Online Access:	http://www.sciencedirect.com/science/article/pii/S2214750018307455

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spelling	doaj-83817d2fc2ff4c1c943165132dea719e2020-11-25T01:55:53ZengElsevierToxicology Reports2214-75002019-01-016897903Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cellsMuhammad Athar Abbasi0Seon-Mi Yu1 Aziz-ur-Rehman2Sabahat Zahra Siddiqui3Song Ja Kim4Hussain Raza5Mubashir Hassan6Abdul Rehman Sadiq Butt7Syed Adnan Ali Shah8Sung-Yum Seo9College of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South Korea; Department of Chemistry, Government College University, Lahore, 54000, Pakistan; Corresponding authors at: College of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South Korea and Department of Chemistry, Government College University, Lahore, 54000, Pakistan.College of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South KoreaDepartment of Chemistry, Government College University, Lahore, 54000, PakistanDepartment of Chemistry, Government College University, Lahore, 54000, PakistanCollege of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South KoreaCollege of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South KoreaInstitute of Molecular Biology and Biotechnology, The University of Lahore, PakistanDepartment of Chemistry, Government College University, Lahore, 54000, PakistanFaculty of Pharmacy and Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA, Cawangan Selangor Kampus, Puncak Alam, 42300 Bandar Puncak Alam, Selangor Darul Ehsan, MalaysiaCollege of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South Korea; Corresponding authors at: College of Natural Sciences, Department of Biological Sciences, Kongju National University, Gongju, 32588, South Korea and Department of Chemistry, Government College University, Lahore, 54000, Pakistan.In the study presented here, a novel chlorobenzylated bi-heterocyclic hybrid molecule (7) was synthesized and its structural confirmation was carried out by IR, 1H-NMR, 13C-NMR and CHN analysis data. This compound 7 was subjected to biological study with B16F10 mouse melanoma cells. The anti-proliferative results showed that 7 showed no significant toxicity at concentrations ranging of 0–44 μM. The treatment of B16F10 cells with 7 at aforementioned concentration range indicated that migration of cells was significantly lower than that of the control cells in a dose dependent manner. The possible migration inhibitory effect of these melanoma cells was further evaluated through gelatinolytic activity of MMP-2 and MMP-9 secreted from B16F10 cells. It was inferred from our results that 7 was not affecting the expression and activity of these enzymes. Some other zinc-dependent matrix metalloproteinases (MMPs) were involved in the inhibitory progression. Taken together, compound 7 inhibited migrations of B16F10 mouse melanoma cells. Therefore, it may deserve consideration as a potential agent for the treatment of cancer. Keywords: 2-Aminothiazole, Triazole, Anti-proliferation, Matrix metalloproteinase, Zymographyhttp://www.sciencedirect.com/science/article/pii/S2214750018307455
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Muhammad Athar Abbasi Seon-Mi Yu Aziz-ur-Rehman Sabahat Zahra Siddiqui Song Ja Kim Hussain Raza Mubashir Hassan Abdul Rehman Sadiq Butt Syed Adnan Ali Shah Sung-Yum Seo
spellingShingle	Muhammad Athar Abbasi Seon-Mi Yu Aziz-ur-Rehman Sabahat Zahra Siddiqui Song Ja Kim Hussain Raza Mubashir Hassan Abdul Rehman Sadiq Butt Syed Adnan Ali Shah Sung-Yum Seo Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells Toxicology Reports
author_facet	Muhammad Athar Abbasi Seon-Mi Yu Aziz-ur-Rehman Sabahat Zahra Siddiqui Song Ja Kim Hussain Raza Mubashir Hassan Abdul Rehman Sadiq Butt Syed Adnan Ali Shah Sung-Yum Seo
author_sort	Muhammad Athar Abbasi
title	Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells
title_short	Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells
title_full	Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells
title_fullStr	Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells
title_full_unstemmed	Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells
title_sort	synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of b16f10 in melanoma cells
publisher	Elsevier
series	Toxicology Reports
issn	2214-7500
publishDate	2019-01-01
description	In the study presented here, a novel chlorobenzylated bi-heterocyclic hybrid molecule (7) was synthesized and its structural confirmation was carried out by IR, 1H-NMR, 13C-NMR and CHN analysis data. This compound 7 was subjected to biological study with B16F10 mouse melanoma cells. The anti-proliferative results showed that 7 showed no significant toxicity at concentrations ranging of 0–44 μM. The treatment of B16F10 cells with 7 at aforementioned concentration range indicated that migration of cells was significantly lower than that of the control cells in a dose dependent manner. The possible migration inhibitory effect of these melanoma cells was further evaluated through gelatinolytic activity of MMP-2 and MMP-9 secreted from B16F10 cells. It was inferred from our results that 7 was not affecting the expression and activity of these enzymes. Some other zinc-dependent matrix metalloproteinases (MMPs) were involved in the inhibitory progression. Taken together, compound 7 inhibited migrations of B16F10 mouse melanoma cells. Therefore, it may deserve consideration as a potential agent for the treatment of cancer. Keywords: 2-Aminothiazole, Triazole, Anti-proliferation, Matrix metalloproteinase, Zymography
url	http://www.sciencedirect.com/science/article/pii/S2214750018307455
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Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells

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