Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry

Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry

Simultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH4HCO3 was used for sample dissolution and trypsin was used for hydrolysis of the gelatins....

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Main Authors: Jia Chen, Xian-Long Cheng, Feng Wei, Qian-Qian Zhang, Ming-Hua Li, Shuang-Cheng Ma
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2015/259757
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spelling doaj-832d54a41e1c4700a5dddc05bbe302952020-11-25T02:43:26ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/259757259757Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass SpectrometryJia Chen0Xian-Long Cheng1Feng Wei2Qian-Qian Zhang3Ming-Hua Li4Shuang-Cheng Ma5National Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, ChinaNational Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, ChinaNational Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, ChinaNational Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, ChinaNational Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, ChinaNational Institute for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing 100050, ChinaSimultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH4HCO3 was used for sample dissolution and trypsin was used for hydrolysis of the gelatins. After separation by a SB-C18 reversed-phase analytical column, collagen marker peptides were detected by mass spectrometry in positive electrospray ionization mode with multiple reaction monitoring. The method was specific, precise and reliable, and suitable for detection of adulterants derived from donkey-hide gelatin and bovine-hide gelatin in deer-horn glue.http://dx.doi.org/10.1155/2015/259757
collection DOAJ
language English
format Article
sources DOAJ
author Jia Chen
Xian-Long Cheng
Feng Wei
Qian-Qian Zhang
Ming-Hua Li
Shuang-Cheng Ma
spellingShingle Jia Chen
Xian-Long Cheng
Feng Wei
Qian-Qian Zhang
Ming-Hua Li
Shuang-Cheng Ma
Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
Journal of Analytical Methods in Chemistry
author_facet Jia Chen
Xian-Long Cheng
Feng Wei
Qian-Qian Zhang
Ming-Hua Li
Shuang-Cheng Ma
author_sort Jia Chen
title Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_short Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_full Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_fullStr Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_full_unstemmed Detection of Gelatin Adulteration in Traditional Chinese Medicine: Analysis of Deer-Horn Glue by Rapid-Resolution Liquid Chromatography-Triple Quadrupole Mass Spectrometry
title_sort detection of gelatin adulteration in traditional chinese medicine: analysis of deer-horn glue by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry
publisher Hindawi Limited
series Journal of Analytical Methods in Chemistry
issn 2090-8865
2090-8873
publishDate 2015-01-01
description Simultaneous identification of donkey-hide gelatin and bovine-hide gelatin in deer-horn glue was established by rapid-resolution liquid chromatography-triple quadrupole mass spectrometry. Water containing 1% NH4HCO3 was used for sample dissolution and trypsin was used for hydrolysis of the gelatins. After separation by a SB-C18 reversed-phase analytical column, collagen marker peptides were detected by mass spectrometry in positive electrospray ionization mode with multiple reaction monitoring. The method was specific, precise and reliable, and suitable for detection of adulterants derived from donkey-hide gelatin and bovine-hide gelatin in deer-horn glue.
url http://dx.doi.org/10.1155/2015/259757
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