EXPRESSION OF SOME ANTIOXIDANT GENES IN SUNFLOWER INFECTED WITH BROOMRAPE

• Main Author: Tatiana Shestakova
• Format: Article
• Language: English
• Published: "Alexandru Ioan Cuza" University of Iași 2015-11-01
• Series: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
• Online Access: http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/1181

<p>Expression levels of ROS-scavenging genes (<em>MnSODI, APX3</em> and <em>AOX1A</em>) in leaves (R5 stage; 90 days after sowing) of seven sunflower genotypes infected with three<em> Orobanche cumana</em> Wallr. populations were assayed in plants with/without broomrape aerial shoots and control group. Five lines were highly susceptible to all three populations. MS-2161A was resistant and MS-2039A was tolerant to broomrape populations. The expression of studied genes was much more altered in highly susceptible genotypes than in those resistant. Significant differences in number of cases of ROS-scavenging genes with modified transcriptional activity in infected and non-symptomatic plants were not ascertained. The transcriptional activity of <em>MnSODI, APX3</em> and <em>AOX1A</em> genes was weakly influenced by infection with broomrape (67 % cases) or was down-regulated (24 % cases). Some up-regulation cases (9 %) for <em>MnSODI</em> (MS-2039) and <em>AOX1A</em> gene (MS-2067) were revealed. <em>AOX1A</em> was the most responsive gene, especially when infection was produced by population from Anenii Noi. </p>