Label-Free Multiple Reaction Monitoring, a Promising Method for Quantification Analyses of Specific Proteins in Bacteria

Label-Free Multiple Reaction Monitoring, a Promising Method for Quantification Analyses of Specific Proteins in Bacteria

<i>Bacillus subtilis</i> produces eight industrially important exo-proteases. For the detection of proteases, the activity- and antibody-based assays are normally used. Current activity-based assays require expensive multiplex chemical substrates which allow specificity determination of...

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Bibliographic Details
Main Authors: Anna A. Toymentseva, Anastasia O. Koryagina, Alexander V. Laikov, Margarita R. Sharipova
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:International Journal of Molecular Sciences
Subjects:
mass spectrometry
multiple reaction monitoring (MRM)
protein quantification
subtilisin-like protease (AprBp)
glutamyl endopeptidase (GseBp)
<i>Bacillus pumilus</i>
Online Access:https://www.mdpi.com/1422-0067/21/14/4924

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