Effects of Different Calcium Silicate Cements on the Inflammatory Response and Odontogenic Differentiation of Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells

• Main Authors: Minsun Chung, Sukjoon Lee, Dongzi Chen, Ukseong Kim, Yaelim Kim, Sunil Kim, Euiseong Kim
• Format: Article
• Language: English
• Published: MDPI AG 2019-04-01
• Series: Materials
• Subjects: calcium silicate cement; human dental pulp stem cell; lipopolysaccharide; inflammation; odontogenic differentiation
• Online Access: https://www.mdpi.com/1996-1944/12/8/1259
• ISSN: 1996-1944

This study aimed to analyze the effects of different calcium silicate cements (CSCs) on the inflammatory response and odontogenic differentiation of lipopolysaccharide-stimulated human dental pulp stem cells. Human dental pulp stem cells (hDPSCs) were stimulated with lipopolysaccharide (LPS) to induce inflammation. These LPS-induced dental pulp stem cells (LDPSCs) were cultured with ProRoot MTA, Biodentine, Retro MTA, and Dycal. Cell viability was evaluated using the Cell Counting Kit-8 assay. Interleukin (IL)-6, IL-8, and transforming growth factor (TGF)-&#946;1 cytokine levels were assessed using the enzyme-linked immunosorbent assay. The expressions of alkaline phosphatase (ALP), osteocalcin, and runt-related transcription factor 2 (RUNX2) were analyzed through real-time polymerase chain reaction. ProRoot MTA, Biodentine, and Retro MTA did not significantly decrease the cell viability of LDPSCs for up to 48 h (<i>p</i> &lt; 0.05). Retro MTA significantly decreased the expression of IL-6 and IL-8 by LDPSCs. ProRoot MTA and Biodentine significantly reduced TGF-&#946; expression by LDPSCs (<i>p</i> &lt; 0.05). Regarding odontogenic differentiation, all CSCs had no effect on ALP expression but increased the production of RUNX2 at 12 h.