Antiproliferative Effect of Chloroform Extract of Cangkring Leaves (Erythrina fusca Lour.) and Its Isolates against HeLa Cells

• Main Authors: Endah Puspitasari, Endah Dwi Setyowati, Binar Asrining Dhiani, Sugeng Riyanto
• Format: Article
• Language: English
• Published: Indonesian Society for Cancer Chemoprevention 2012-02-01
• Series: ISCC (Indonesian Journal of Cancer Chemoprevention)
• Online Access: https://ijcc.chemoprev.org/index.php/ijcc/article/view/56

Cervical cancer is a type of cancer possessing the 3rd highest incidence among women worldwide. Today’s therapies against cancer are still ineffective, thus people are seeking for alternative method. Erythrina fusca Lour. has been used traditionally as anticancer. This experiment is conducted to study the ability of chloroform extract of E. fusca Lour. leaves and its isolates on HeLa cervical cancer cells. The cytotoxicity assay and doubling time assay were done using direct counting method, while the DNA staining assay was done using acrydine orange. The chloroform extract and isolate # 30 showed cytotoxic activity with IC50 of 16 and 5 µg/ml, respectively, while isolate # 2 and isolate # 8 didn’t. The effluent # 30 could not affect HeLa cells growth at the given concentration, but it might promote DNA fragmentation indicating apoptosis induction. The molecular mechanisms underlying these effects still need to be further explored. Keywords: Erythrina fusca Lour., isolate and effluent of chloroform extract, antiproliferative, cervical cancer