Mechanisms of EGF Regulation of COX-2 Through the STAT5 Signaling Pathway in Human Lung Adenocarcinoma A549 Cells

• Main Authors: Jian CUI, Yubo YAN, Yanzhong XIN, Jiyao LI, Qing DONG, Guibin ZHAO, Jingquan HAN, Shouqiang CAO
• Format: Article
• Language: zho
• Published: Chinese Anti-Cancer Association; Chinese Antituberculosis Association 2013-04-01
• Series: Chinese Journal of Lung Cancer
• Subjects: STAT5; COX-2; Lung neoplasms; A549; EGF
• Online Access: http://dx.doi.org/10.3779/j.issn.1009-3419.2013.04.01

Background and objective It has been proved that cyclooxygenase-2 (COX-2) is a key factor in lung cancer oncogenesis. COX-2 can be induced by a number of cytokines and growth factors and can be regulated by the JAK/STAT signaling pathway. Inhibiting the expression of COX-2 can prevent the development of lung cancer. The aim fo this study is to investigate whether the epidermal growth factor (EGF) can stimulate the signal transducers and activators of transcription 5 (STAT5) as well as to discover the effects of the STAT5 signaling pathway on the COX-2 in human lung adenocarcinoma A549 cells. Methods The phenomenon of STAT5 activation stimulated by the EGF was assayed through immunofluorescence and Western blot. The adenovirus containing the wild-type (WT)-STAT5 (AdWT-STAT5) plasmid, dominant-negative (DN)-STAT5 (Ad-CMV5Stat5aΔ740) plasmid, and STAT5 siRNA were transfected into A549 cells. The latter two groups were stimulated using EGF. Reverse transcriptase polymerase chain reaction was used to detect the mRNA expression of COX-2. Results STAT5 was not activated in A549 cells in vitro. EGF stimulation significantly increased the level of the p-STAT5 protein and induces the shuttling of p-STAT5 from the cytoplasm into the nucleus. STAT5 activation was crucial for the COX-2 expression induced by the EGF. STAT5 was required for COX-2 expression, but can mediated the effects of the COX-2 expression through pathways that were independent of transcriptional activation. Conclusion COX-2 expression is dependent on STAT5 phosphorylation. A second pathway does not require STAT5 phosphorylation.