Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse.
Recent studies have shown that oxidative stress contributes to the pathogenesis of muscle damage in dystrophic (mdx) mice. In this study we have investigated the role of NADPH oxidase as a source of the oxidative stress in these mice. The NADPH oxidase subunits gp91(phox), p67(phox) and rac 1 were i...
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2010-12-01
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doaj-826629b012794c32807243f67805b67e2020-11-25T00:23:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-12-01512e1535410.1371/journal.pone.0015354Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse.Nicholas P WhiteheadElla W YeungStanley C FroehnerDavid G AllenRecent studies have shown that oxidative stress contributes to the pathogenesis of muscle damage in dystrophic (mdx) mice. In this study we have investigated the role of NADPH oxidase as a source of the oxidative stress in these mice. The NADPH oxidase subunits gp91(phox), p67(phox) and rac 1 were increased 2-3 fold in tibilais anterior muscles from mdx mice compared to wild type. Importantly, this increase occurred in 19 day old mice, before the onset of muscle necrosis and inflammation, suggesting that NADPH oxidase is an important source of oxidative stress in mdx muscle. In muscles from 9 week old mdx mice, gp91(phox) and p67(phox) were increased 3-4 fold and NADPH oxidase superoxide production was 2 times greater than wild type. In single fibers from mdx muscle NADPH oxidase subunits were all located on or near the sarcolemma, except for p67(phox),which was expressed in the cytosol. Pharmacological inhibition of NADPH oxidase significantly reduced the intracellular Ca(2+) rise following stretched contractions in mdx single fibers, and also attenuated the loss of muscle force. These results suggest that NADPH oxidase is a major source of reactive oxygen species in dystrophic muscle and its enhanced activity has a stimulatory effect on stretch-induced Ca(2+) entry, a key mechanism for muscle damage and functional impairment.http://europepmc.org/articles/PMC3004864?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nicholas P Whitehead Ella W Yeung Stanley C Froehner David G Allen |
spellingShingle |
Nicholas P Whitehead Ella W Yeung Stanley C Froehner David G Allen Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse. PLoS ONE |
author_facet |
Nicholas P Whitehead Ella W Yeung Stanley C Froehner David G Allen |
author_sort |
Nicholas P Whitehead |
title |
Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse. |
title_short |
Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse. |
title_full |
Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse. |
title_fullStr |
Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse. |
title_full_unstemmed |
Skeletal muscle NADPH oxidase is increased and triggers stretch-induced damage in the mdx mouse. |
title_sort |
skeletal muscle nadph oxidase is increased and triggers stretch-induced damage in the mdx mouse. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-12-01 |
description |
Recent studies have shown that oxidative stress contributes to the pathogenesis of muscle damage in dystrophic (mdx) mice. In this study we have investigated the role of NADPH oxidase as a source of the oxidative stress in these mice. The NADPH oxidase subunits gp91(phox), p67(phox) and rac 1 were increased 2-3 fold in tibilais anterior muscles from mdx mice compared to wild type. Importantly, this increase occurred in 19 day old mice, before the onset of muscle necrosis and inflammation, suggesting that NADPH oxidase is an important source of oxidative stress in mdx muscle. In muscles from 9 week old mdx mice, gp91(phox) and p67(phox) were increased 3-4 fold and NADPH oxidase superoxide production was 2 times greater than wild type. In single fibers from mdx muscle NADPH oxidase subunits were all located on or near the sarcolemma, except for p67(phox),which was expressed in the cytosol. Pharmacological inhibition of NADPH oxidase significantly reduced the intracellular Ca(2+) rise following stretched contractions in mdx single fibers, and also attenuated the loss of muscle force. These results suggest that NADPH oxidase is a major source of reactive oxygen species in dystrophic muscle and its enhanced activity has a stimulatory effect on stretch-induced Ca(2+) entry, a key mechanism for muscle damage and functional impairment. |
url |
http://europepmc.org/articles/PMC3004864?pdf=render |
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