Growth inhibition of human retinoblastoma by miR-218 <i>via</i> down-regulation of Bmi-1 expression

• Main Authors: Lin-Sheng Yang, Li-Lun Wang, Qing-Wei Du
• Format: Article
• Language: English
• Published: Press of International Journal of Ophthalmology (IJO PRESS) 2015-12-01
• Series: Guoji Yanke Zazhi
• Subjects: miR-218; retinoblastoma; proliferation; apoptosis; Bmi-1
• Online Access: http://ies.ijo.cn/cn_publish/2015/12/201512005.pdf

AIM:To investigate the level of miR-218 in human retinoblastoma(RB)and its effect on the potential mechanism of tumorgenesis. <p>METHODS:Real-time PCR was applied to detect the expression of miR-218 in human RB and the corresponding tumor-adjacent tissues to analyze the relation between miR-218 and clinic pathology characteristics. The artificial miR-218 was transiently transfected into human Y79 cells in <i>vitro</i>. The proliferation and apoptosis of the cells were detected by MTT assay and flow cytometry, respectively. The expression level of Bmi-1 mRNA and protein were determined by RT-PCR and Western blot. <p>RESULTS:The expression level of miR-218 in RB tissues was significantly lower than that in the adjacent tissues, and it was associated with optic nerve infiltration and differentiated degree. Over-expressed miR-218 in Y79 cells suppressed cell proliferation and promoted cell apoptosis, and down-regulated mRNA and protein expressions of Bmi-1. <p>CONCLUSION:The expression of miR-218 in RB tissues is significantly lower than that in tumor-adjacent tissues.MiR-218 could inhibit RB cell proliferation and induce apoptosis by down-regulating the expression of Bmi-1.