Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus

<p>Abstract</p> <p>To establish an accurate, rapid, and a quantifiable method for the detection of the newly emerged duck Tembusu virus (DTMUV) that recently caused a widespread infectious disease in ducks in China, we developed a TaqMan-based real-time PCR assay by using E gene-sp...

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Main Authors: Yan Liping, Yan Pixi, Zhou Jiewen, Teng Qiaoyang, Li Zejun
Format: Article
Language:English
Published: BMC 2011-10-01
Series:Virology Journal
Online Access:http://www.virologyj.com/content/8/1/464
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spelling doaj-8257877173f74e4bb14f99f74b13f2552020-11-24T21:22:36ZengBMCVirology Journal1743-422X2011-10-018146410.1186/1743-422X-8-464Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virusYan LipingYan PixiZhou JiewenTeng QiaoyangLi Zejun<p>Abstract</p> <p>To establish an accurate, rapid, and a quantifiable method for the detection of the newly emerged duck Tembusu virus (DTMUV) that recently caused a widespread infectious disease in ducks in China, we developed a TaqMan-based real-time PCR assay by using E gene-specific primers and a TaqMan probe. This real-time PCR assay was 100 times more sensitive than the conventional PCR. The reproducibility and specificity of the real-time PCR assay were confirmed using plasmids containing E genes or RNAs and DNAs extracted from well-known viruses causing duck diseases. The reliability of this real-time PCR assay was confirmed in 19 of the 24 swab samples, 22 of the 24 tissue samples collected from experimentally infected ducks, as well as 15 of the 21 clinical samples collected from sick ducks since they were verified as DTMUV-positive. The results reveal that the newly established real-time PCR assay might be a useful diagnostic method for epidemiologically investigating and closely observing the newly emerged DTMUV.</p> http://www.virologyj.com/content/8/1/464
collection DOAJ
language English
format Article
sources DOAJ
author Yan Liping
Yan Pixi
Zhou Jiewen
Teng Qiaoyang
Li Zejun
spellingShingle Yan Liping
Yan Pixi
Zhou Jiewen
Teng Qiaoyang
Li Zejun
Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
Virology Journal
author_facet Yan Liping
Yan Pixi
Zhou Jiewen
Teng Qiaoyang
Li Zejun
author_sort Yan Liping
title Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
title_short Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
title_full Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
title_fullStr Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
title_full_unstemmed Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
title_sort establishing a taqman-based real-time pcr assay for the rapid detection and quantification of the newly emerged duck tembusu virus
publisher BMC
series Virology Journal
issn 1743-422X
publishDate 2011-10-01
description <p>Abstract</p> <p>To establish an accurate, rapid, and a quantifiable method for the detection of the newly emerged duck Tembusu virus (DTMUV) that recently caused a widespread infectious disease in ducks in China, we developed a TaqMan-based real-time PCR assay by using E gene-specific primers and a TaqMan probe. This real-time PCR assay was 100 times more sensitive than the conventional PCR. The reproducibility and specificity of the real-time PCR assay were confirmed using plasmids containing E genes or RNAs and DNAs extracted from well-known viruses causing duck diseases. The reliability of this real-time PCR assay was confirmed in 19 of the 24 swab samples, 22 of the 24 tissue samples collected from experimentally infected ducks, as well as 15 of the 21 clinical samples collected from sick ducks since they were verified as DTMUV-positive. The results reveal that the newly established real-time PCR assay might be a useful diagnostic method for epidemiologically investigating and closely observing the newly emerged DTMUV.</p>
url http://www.virologyj.com/content/8/1/464
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AT yanpixi establishingataqmanbasedrealtimepcrassayfortherapiddetectionandquantificationofthenewlyemergedducktembusuvirus
AT zhoujiewen establishingataqmanbasedrealtimepcrassayfortherapiddetectionandquantificationofthenewlyemergedducktembusuvirus
AT tengqiaoyang establishingataqmanbasedrealtimepcrassayfortherapiddetectionandquantificationofthenewlyemergedducktembusuvirus
AT lizejun establishingataqmanbasedrealtimepcrassayfortherapiddetectionandquantificationofthenewlyemergedducktembusuvirus
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