Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research.

Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding genes are often required. We developed a novel...

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Bibliographic Details
Main Authors: Mostafa Aboulela, Yuji Tanaka, Kohji Nishimura, Shoji Mano, Mikio Nishimura, Sumie Ishiguro, Tetsuya Kimura, Tsuyoshi Nakagawa
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5433782?pdf=render