Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex

Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex

Trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression o...

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Main Authors: E. Łopieńska-Biernat, E. A. Zaobidna, M. Dmitryjuk
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Journal of Parasitology Research
Online Access:http://dx.doi.org/10.1155/2015/438145
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spelling doaj-8233434ba618407e9f72bc751fe541cd2020-11-24T22:50:34ZengHindawi LimitedJournal of Parasitology Research2090-00232090-00312015-01-01201510.1155/2015/438145438145Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplexE. Łopieńska-Biernat0E. A. Zaobidna1M. Dmitryjuk2Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-917 Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-917 Olsztyn, PolandDepartment of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A, 10-917 Olsztyn, PolandTrehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen—trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP)—in stage L3 and stage L4 larvae of A. simplex. The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction. The A. simplex ribosomal gene (18S) was used as a reference gene. Enzymatic activity was determined. The expression of trehalose enzyme genes was higher in L3 than in L4 larvae, but an inverse relationship was noted for the expression of gs and gp genes.http://dx.doi.org/10.1155/2015/438145
collection DOAJ
language English
format Article
sources DOAJ
author E. Łopieńska-Biernat
E. A. Zaobidna
M. Dmitryjuk
spellingShingle E. Łopieńska-Biernat
E. A. Zaobidna
M. Dmitryjuk
Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
Journal of Parasitology Research
author_facet E. Łopieńska-Biernat
E. A. Zaobidna
M. Dmitryjuk
author_sort E. Łopieńska-Biernat
title Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
title_short Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
title_full Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
title_fullStr Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
title_full_unstemmed Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
title_sort expression of genes encoding the enzymes for glycogen and trehalose metabolism in l3 and l4 larvae of anisakis simplex
publisher Hindawi Limited
series Journal of Parasitology Research
issn 2090-0023
2090-0031
publishDate 2015-01-01
description Trehalose and glycogen metabolism plays an important role in supporting life processes in many nematodes, including Anisakis simplex. Nematodes, cosmopolitan helminths parasitizing sea mammals and humans, cause a disease known as anisakiasis. The aim of this study was to investigate the expression of genes encoding the enzymes involved in the metabolism of trehalose and glycogen—trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP)—in stage L3 and stage L4 larvae of A. simplex. The expression of mRNA all four genes, tps, tpp, gs, and gp, was examined by real-time polymerase chain reaction. The A. simplex ribosomal gene (18S) was used as a reference gene. Enzymatic activity was determined. The expression of trehalose enzyme genes was higher in L3 than in L4 larvae, but an inverse relationship was noted for the expression of gs and gp genes.
url http://dx.doi.org/10.1155/2015/438145
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AT eazaobidna expressionofgenesencodingtheenzymesforglycogenandtrehalosemetabolisminl3andl4larvaeofanisakissimplex
AT mdmitryjuk expressionofgenesencodingtheenzymesforglycogenandtrehalosemetabolisminl3andl4larvaeofanisakissimplex
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