Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity

Deubiquitinating enzymes (DUBs) are critical regulators of cellular processes by removing ubiquitin from specific targets. Here global kinetic modelling reveals the mechanism by which the low intrinsic activity of USP7 is substantially enhanced on a specific physiological target.

Bibliographic Details
Main Authors:	Robbert Q. Kim, Paul P. Geurink, Monique P. C. Mulder, Alexander Fish, Reggy Ekkebus, Farid El Oualid, Willem J. van Dijk, Duco van Dalen, Huib Ovaa, Hugo van Ingen, Titia K. Sixma
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-01-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-08231-5

id	doaj-822bd4cb8eb04f3199afc82488ed53d3
record_format	Article
spelling	doaj-822bd4cb8eb04f3199afc82488ed53d32021-05-11T11:42:20ZengNature Publishing GroupNature Communications2041-17232019-01-0110111610.1038/s41467-018-08231-5Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activityRobbert Q. Kim0Paul P. Geurink1Monique P. C. Mulder2Alexander Fish3Reggy Ekkebus4Farid El Oualid5Willem J. van Dijk6Duco van Dalen7Huib Ovaa8Hugo van Ingen9Titia K. Sixma10Division of Biochemistry and Oncode Institute, Netherlands Cancer InstituteDivision of Cell Biology II, Netherlands Cancer InstituteDivision of Cell Biology II, Netherlands Cancer InstituteDivision of Biochemistry and Oncode Institute, Netherlands Cancer InstituteDivision of Cell Biology II, Netherlands Cancer InstituteUbiQ Bio BV, Science Park 408Division of Biochemistry and Oncode Institute, Netherlands Cancer InstituteDivision of Cell Biology II, Netherlands Cancer InstituteDivision of Cell Biology II, Netherlands Cancer InstituteMacromolecular Biochemistry, Leiden Institute of Chemistry, Leiden UniversityDivision of Biochemistry and Oncode Institute, Netherlands Cancer InstituteDeubiquitinating enzymes (DUBs) are critical regulators of cellular processes by removing ubiquitin from specific targets. Here global kinetic modelling reveals the mechanism by which the low intrinsic activity of USP7 is substantially enhanced on a specific physiological target.https://doi.org/10.1038/s41467-018-08231-5
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Robbert Q. Kim Paul P. Geurink Monique P. C. Mulder Alexander Fish Reggy Ekkebus Farid El Oualid Willem J. van Dijk Duco van Dalen Huib Ovaa Hugo van Ingen Titia K. Sixma
spellingShingle	Robbert Q. Kim Paul P. Geurink Monique P. C. Mulder Alexander Fish Reggy Ekkebus Farid El Oualid Willem J. van Dijk Duco van Dalen Huib Ovaa Hugo van Ingen Titia K. Sixma Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity Nature Communications
author_facet	Robbert Q. Kim Paul P. Geurink Monique P. C. Mulder Alexander Fish Reggy Ekkebus Farid El Oualid Willem J. van Dijk Duco van Dalen Huib Ovaa Hugo van Ingen Titia K. Sixma
author_sort	Robbert Q. Kim
title	Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity
title_short	Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity
title_full	Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity
title_fullStr	Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity
title_full_unstemmed	Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity
title_sort	kinetic analysis of multistep usp7 mechanism shows critical role for target protein in activity
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-01-01
description	Deubiquitinating enzymes (DUBs) are critical regulators of cellular processes by removing ubiquitin from specific targets. Here global kinetic modelling reveals the mechanism by which the low intrinsic activity of USP7 is substantially enhanced on a specific physiological target.
url	https://doi.org/10.1038/s41467-018-08231-5
work_keys_str_mv	AT robbertqkim kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT paulpgeurink kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT moniquepcmulder kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT alexanderfish kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT reggyekkebus kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT farideloualid kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT willemjvandijk kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT ducovandalen kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT huibovaa kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT hugovaningen kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity AT titiaksixma kineticanalysisofmultistepusp7mechanismshowscriticalrolefortargetproteininactivity
_version_	1721446082584510464

Kinetic analysis of multistep USP7 mechanism shows critical role for target protein in activity

Similar Items