A Plausible Antibacterial Green Synthesized AgNPs from Tridax procumbens Leaf-flower Extract

• Main Authors: Jesse Joel T., Jesvin Shobini
• Format: Article
• Language: English
• Published: Journal of Pure and Applied Microbiology 2018-12-01
• Series: Journal of Pure and Applied Microbiology
• Subjects: antibacterial; green synthesis; tridax procumbens; leaf flower
• Online Access: https://microbiologyjournal.org/a-plausible-antibacterial-green-synthesized-agnps-from-tridax-procumbens-leaf-flower-extract/

The current study was aimed at the green synthesis of silver nanoparticles (AgNPs) from leaf-flower and flower extracts of Tridax procumbens which are effective against oral pathogens and its characterization. The silver nanoparticle attaches to the cell membrane of bacteria and disturbs its function by penetrating inside bacterial cell by releasing silver ions and inhibits the bacterial growth. The silver nanoparticles obtained from the bio-reduction method using leaf-flower and flower extract with silver nitrate was characterized using UV- visible spectroscopy (UV-Vis), X-Ray Diffraction (XRD), Fourier Transform Infra-Red spectroscopy (FTIR), Scanning Electron Microscope (SEM). The presence of AgNPs in the aqueous extract was confirmed by the yellowish-brown color change of the extract and by Surface Plasmon Resonance (SPR) peaks in the UV- Visible spectroscopy (430nm-510nm) analysis, X-Ray Diffraction (XRD) for Functional groups, Fourier Transform Infra-Red spectroscopy (FTIR) for Phytophenols (3448.72/cm). Scanning Electron Microscope (SEM) showed particle range 33-45nm and particle size analysis using Zeta potential (142.2 (d.nm). The other analysis showed that the AgNPs were irregularly shaped, crystalline in nature and less stabilized due the effect of capping agents in the extracts. In this study, the antibacterial activity of these nanoparticles was tested against the oral pathogens. The antibiotic resistance in pathogenic microbes has led to the emergence of multidrug-resistant (MDR) pathogens. Therefore, the Leaf-flower combination was seen as plausible antibacterial agent in the prevention of Dental caries and can be used as an additive in Tooth paste or mouth freshners.