CD11c-specific bio-nanocapsule enhances vaccine immunogenicity by targeting immune cells

• Main Authors: Hidenori Matsuo, Masaharu Somiya, Masumi Iijima, Takeshi Arakawa, Shun’ichi Kuroda
• Format: Article
• Language: English
• Published: BMC 2018-08-01
• Series: Journal of Nanobiotechnology
• Subjects: Bio-nanocapsule; Hepatitis B virus; Vaccine; Dendritic cell; In vivo targeting; Protective immunity
• Online Access: http://link.springer.com/article/10.1186/s12951-018-0386-6

Abstract Background Various nanocarriers have been used to deliver subunit vaccines specifically to dendritic cells (DCs) for the improvement of immunogenicity. However, due to their insufficient DC priming ability, these vaccines could not elicit effective innate immunity. We have recently developed a DC-targeting bio-nanocapsule (BNC) by displaying anti-CD11c IgGs via protein A-derived IgG Fc-binding Z domain on the hepatitis B virus envelope L protein particles (α-DC-ZZ-BNC). Results After the chemical modification with antigens (Ags), the α-DC-ZZ-BNC-Ag complex could deliver Ags to DCs efficiently, leading to effective DC maturation and efficient endosomal escape of Ags, followed by Ag-specific T cell responses and IgG productions. Moreover, the α-DC-ZZ-BNC modified with Japanese encephalitis virus (JEV) envelope-derived D3 Ags could confer protection against 50-fold lethal dose of JEV injection on mice. Conclusion The α-DC-ZZ-BNC-Ag platform was shown to induce humoral and cellular immunities effectively without any adjuvant.