Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies.

Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies.

Screening of antibody (Ab) libraries by direct display on the surface of E. coli cells is hampered by the presence of the outer membrane (OM). In this work we demonstrate that the native β-domains of EhaA autotransporter and intimin, two proteins from enterohemorrhagic E. coli O157:H7 (EHEC) with op...

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Bibliographic Details
Main Authors: Valencio Salema, Elvira Marín, Rocio Martínez-Arteaga, David Ruano-Gallego, Sofía Fraile, Yago Margolles, Xema Teira, Carlos Gutierrez, Gustavo Bodelón, Luis Ángel Fernández
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3781032?pdf=render

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