Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood

Immune profiles in endometrium may be changed in patients with IVF failure and its possible correlations with immune parameters in peripheral blood are important for the diagnostic approach. Such correlations in healthy women are unknown and have been studied in the present research. The expression...

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Main Authors: Viktor Chernyshov, Boris Dons’koi, Iryna Sudoma, Yana Goncharova
Format: Article
Language:English
Published: Termedia Publishing House 2019-09-01
Series:Central European Journal of Immunology
Subjects:
Online Access:https://www.termedia.pl/Comparison-of-T-and-NK-lymphocyte-subsets-between-human-endometrial-tissue-and-peripheral-blood,10,38546,1,1.html
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spelling doaj-81a8a5c5e9d5400a843d073f03107e132020-11-25T02:03:45ZengTermedia Publishing HouseCentral European Journal of Immunology1426-39121644-41242019-09-0144331632110.5114/ceji.2019.8961038546Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral bloodViktor ChernyshovBoris Dons’koiIryna SudomaYana GoncharovaImmune profiles in endometrium may be changed in patients with IVF failure and its possible correlations with immune parameters in peripheral blood are important for the diagnostic approach. Such correlations in healthy women are unknown and have been studied in the present research. The expression of CD56, CD158a, HLA DR, CD69 in T lymphocytes, CD4 T lymphocytes, CD8+ T lymphocytes and NK cells were studied by flow-cytometry in endometrium and peripheral blood in healthy 24 donors of oocytes aged 25-32 years. Levels of T lymphocyte and T helper cells were lower in endometrium and no differences in CD8 T lymphocytes were registered between endometrium and peripheral blood. The expression of HLA DR and especially CD69 was higher in CD3, CD4, CD8 T cells in endometrium in comparison with peripheral blood. The endometrium lymphocyte population was enriched by NK cells that were generally CD56++ with a higher expression of HLA DR and almost in total were CD69 positive. Strong positive correlations of CD8 expression in NK cells (r = 0.6478, p < 0.001) and HLA DR expression in CD8 T cells (r = 0.6107, p < 0.01) between peripheral blood and endometrium were registered in fertile women. The endometrial CD56 expression in CD8+ T cells negatively correlated with endometrial CD8 expression in NK cells (r = –0.5252, p < 0.01) which possibly reflected a suppressive and regulating mechanism in the endometrium. CD8+ NK cells and HLA DR+ CD8 T cells in endometrium were related to the same subsets in peripheral blood.https://www.termedia.pl/Comparison-of-T-and-NK-lymphocyte-subsets-between-human-endometrial-tissue-and-peripheral-blood,10,38546,1,1.htmlendometrium peripheral blood immune profiles
collection DOAJ
language English
format Article
sources DOAJ
author Viktor Chernyshov
Boris Dons’koi
Iryna Sudoma
Yana Goncharova
spellingShingle Viktor Chernyshov
Boris Dons’koi
Iryna Sudoma
Yana Goncharova
Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood
Central European Journal of Immunology
endometrium
peripheral blood
immune profiles
author_facet Viktor Chernyshov
Boris Dons’koi
Iryna Sudoma
Yana Goncharova
author_sort Viktor Chernyshov
title Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood
title_short Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood
title_full Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood
title_fullStr Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood
title_full_unstemmed Comparison of T and NK lymphocyte subsets between human endometrial tissue and peripheral blood
title_sort comparison of t and nk lymphocyte subsets between human endometrial tissue and peripheral blood
publisher Termedia Publishing House
series Central European Journal of Immunology
issn 1426-3912
1644-4124
publishDate 2019-09-01
description Immune profiles in endometrium may be changed in patients with IVF failure and its possible correlations with immune parameters in peripheral blood are important for the diagnostic approach. Such correlations in healthy women are unknown and have been studied in the present research. The expression of CD56, CD158a, HLA DR, CD69 in T lymphocytes, CD4 T lymphocytes, CD8+ T lymphocytes and NK cells were studied by flow-cytometry in endometrium and peripheral blood in healthy 24 donors of oocytes aged 25-32 years. Levels of T lymphocyte and T helper cells were lower in endometrium and no differences in CD8 T lymphocytes were registered between endometrium and peripheral blood. The expression of HLA DR and especially CD69 was higher in CD3, CD4, CD8 T cells in endometrium in comparison with peripheral blood. The endometrium lymphocyte population was enriched by NK cells that were generally CD56++ with a higher expression of HLA DR and almost in total were CD69 positive. Strong positive correlations of CD8 expression in NK cells (r = 0.6478, p < 0.001) and HLA DR expression in CD8 T cells (r = 0.6107, p < 0.01) between peripheral blood and endometrium were registered in fertile women. The endometrial CD56 expression in CD8+ T cells negatively correlated with endometrial CD8 expression in NK cells (r = –0.5252, p < 0.01) which possibly reflected a suppressive and regulating mechanism in the endometrium. CD8+ NK cells and HLA DR+ CD8 T cells in endometrium were related to the same subsets in peripheral blood.
topic endometrium
peripheral blood
immune profiles
url https://www.termedia.pl/Comparison-of-T-and-NK-lymphocyte-subsets-between-human-endometrial-tissue-and-peripheral-blood,10,38546,1,1.html
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