Extraction, Purification and Primary Characterization of Polysaccharides from Defatted Peanut (Arachis hypogaea) Cakes

• Main Authors: Hongzhi Liu, Nan Jiang, Li Liu, Xiaojing Sheng, Aimin Shi, Hui Hu, Ying Yang, Qiang Wang
• Format: Article
• Language: English
• Published: MDPI AG 2016-06-01
• Series: Molecules
• Subjects: defatted peanut cake; polysaccharide; response surface methodology; purification and characterization
• Online Access: http://www.mdpi.com/1420-3049/21/6/716
• ISSN: 1420-3049

The hot-water extraction, purification and characterization of polysaccharides from defatted peanut cake (PPC) were investigated in this study. A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X1), extraction time (X2) and ratio of water to raw material (X3). The optimum conditions were 85 °C, 3 h and 20:1 (mL/g) respectively. Regression analysis was done to reveal the experimental results which include 34.97% extraction rate while the value verified under these conditions was 34.49%. The crude PPC was sequentially further purified by Sephadex G-100 chromatography, and one purified fraction was obtained. The PPC purified fraction was characterized by FT-IR, HPAEC; SEC-MALLS. The average molecular weight of the PPC purified fraction was 2.383 × 105 Da. The polysaccharide was mainly composed of glucose, galactose, arabinose and xylose. The PPC have the typical absorption of polysaccharide.