Identification and developmental expression of putative gene encoding juvenile hormone esterase (CpJHE-like) in codling moth, Cydia pomonella (L.)

• Main Authors: Cong HUANG, Qiang WU, Chun-yan JIANG, Long-sheng XING, Guo-liang SHI, Bin ZHANG, Wan-qiang QIAN, You-zhi LI, Yu XI, Nian-wan YANG, Fang-hao WAN
• Format: Article
• Language: English
• Published: Elsevier 2019-07-01
• Series: Journal of Integrative Agriculture
• Subjects: juvenile hormone esterase; CpJHE-like; codling moth; Cydia pomonella
• Online Access: http://www.sciencedirect.com/science/article/pii/S2095311919626821

Juvenile hormone esterase (JHE) is a key enzyme for insects, playing an important role in the regulation of insect growth, development, diapause and reproduction. We identified a complete putative JHE of Cydia pomonella (CpJHE-like) which is comprised of a 1 761 bp coding sequence (CDS) encoding 587 amino acid residues from the transcriptome data. The deduced protein sequence of CpJHE-like showed the highest identity of 60.44% with the Adoxophyes honmai JHE (AhJHE) and the minimal identity of 25.81% with Aedes aegypti JHE (AaJHE). CpJHE-like exhibited all the seven typical motifs of the functional JHEs and had the highly consistent tertiary structure with Manduca sexta JHE (MsJHE). Phylogenetic analysis showed that the CpJHE-like was close to two JHEs from the family Tortricidae. The CpJHE-like transcript level take a leap in the 3-day-old fifth instar larva, increased about 300-fold compared to the basal level. Tissue-specific expression profile showed that the CpJHE-like transcript was expressed mainly in the fat body. This study indicates that the CpJHE-like is the functional JHE, which may play vital roles in the development and reproduction of C. pomonella.