Comparative analysis of the eff ectiveness of analytical methods for the determination of afl atoxins in milk and dairy products (review information)

• Main Authors: Senin S., Danchuk V., Midyk S., Ushkalov V., Iakubchak О.
• Format: Article
• Language: English
• Published: Bila Tserkva National Agrarian University 2020-11-01
• Series: Науковий вісник ветеринарної медицини
• Subjects: mycotoxins; raw milk; analytical methods; quechers.
• Online Access: http://nvvm.btsau.edu.ua/sites/default/files/visnyky/vet/senin_2_2020_0.pdf

The dairy industry of Ukraine is developing dynamically, its needs for the quality of raw materials are growing signifi cantly. Detection of mycotoxins in raw milk is one of the main indicators of its safety. The high degree of toxicity of mycotoxins is a threat to the health of the lactating animal, so a large number of them are excreted in milk. If we talk about ruminants, the vast majority of mycotoxins are utilized by microorganisms of the pancreas, which does not occur in monogastric animals, so the list of mycotoxins in their milk can be much wider than the secretion of mammalian mammals. To date, the maximum permissible levels (MRLs) of mycotoxins in raw milk and dairy products have been established. Thus, a comprehensive determination of the content of mycotoxins in the secretion of the breast has not only technological but also important diagnostic value. Milk sample preparation is the most important step in the determinati on of mycotoxins and consists of sampling, extraction and purifi cation from impurities. For the extraction of afl atoxins, the method of liquid extraction wit h acetonitrile or chloroform is used. Purifi cation of extracts is carried out on immunoaffi nity columns, cartridges with special sorbents or using certain manufacturers (MycoSep®).Enzyme-linked immunosorbent assay and high-performance liquid chromatography with fl uorescence detection are used to determine afl atoxin B1 and M1 in raw milk of cows. However, all these methods have a number of disadvantages, namely: lo ng and expensive sample preparation and insuffi ciently high selectivity. Currently, the complex determination of mycotoxins in various matrices by high-performance liquid chromatography with mass spectrometric detection (LC-MS/MS) and the use of modifi ed QuEChERS sample preparation is gaining popularity. The advantage of this technique is the combination of faster and cheaper sample preparation of QuEChERS samples with highly selective LC-MS/MS chromatography.