Development of Microsatellite Markers for the Coastal Shrub <i>Scaevola taccada</i> (Goodeniaceae)

• Main Authors: Haruko Ando, Naoko Emura, Tetsuro Denda, Naoyuki Nakahama, Miho Inoue-Murayama, Yuji Isagi
• Format: Article
• Language: English
• Published: Wiley 2014-04-01
• Series: Applications in Plant Sciences
• Subjects: 454 sequencing; genetic structure; Goodeniaceae; Scaevola taccada; seed dispersal
• Online Access: http://www.bioone.org/doi/full/10.3732/apps.1300094

Premise of the study: Microsatellite markers were developed for the coastal shrub species <i>Scaevola taccada</i> to estimate the population genetic structure, which may reflect different seed dispersal patterns. Methods and Results: Thirteen microsatellite primer sets were developed for <i>S. taccada</i> using 454 pyrosequencing. The primer sets were tested on 64 individuals sampled from two populations in Japan. Fragments were amplified using the primers, with one to 10 alleles per locus, and the expected heterozygosity ranged from 0.00 to 0.85. Conclusions: These results indicate the utility of markers in <i>S. taccada</i> for broad estimations of the population genetic structure of this species.