Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Identification of active compounds

• Main Authors: Jana Van Dycke, Jasper Rymenants, Johan Neyts, Joana Rocha-Pereira
• Format: Article
• Language: English
• Published: SAGE Publishing 2021-06-01
• Series: Antiviral Chemistry & Chemotherapy
• Online Access: https://doi.org/10.1177/20402066211026852

Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. As potent and safe anti-norovirus small molecules are urgently needed, we here describe the detailed protocol for a set of assays that will allow the identification of novel norovirus inhibitors.