Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury

Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury

We examined the role of hydrogen peroxide production in cyclosporine A (CsA)-induced LLC-PK1 injury. After exposure to CsA (0.1 μM – 100 μM), cytotoxicity assessed by lactate dehydrogenase release to the media increased dose-dependently. LLC-PK1 cells produced hydrogen peroxide, visualized by 2,7-di...

Full description

Bibliographic Details
Main Authors: Masashi Nishida, Hiroshi Ogawa, Megumi Tamai, Kohhei Ishiwari, Kenji Hamaoka
Format: Article
Language:English
Published: Elsevier 2003-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319327410
id doaj-80f1f028f1e64c50ae87f8867f52fe3a
record_format Article
spelling doaj-80f1f028f1e64c50ae87f8867f52fe3a2020-11-25T01:10:31ZengElsevierJournal of Pharmacological Sciences1347-86132003-01-01913255258Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) InjuryMasashi Nishida0Hiroshi Ogawa1Megumi Tamai2Kohhei Ishiwari3Kenji Hamaoka4Division of Pediatrics, Children’s Research Hospital, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, JapanDepartment of Pediatrics, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, JapanDepartment of Pediatrics, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, JapanDivision of Pediatrics, Children’s Research Hospital, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, JapanDivision of Pediatrics, Children’s Research Hospital, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, JapanWe examined the role of hydrogen peroxide production in cyclosporine A (CsA)-induced LLC-PK1 injury. After exposure to CsA (0.1 μM – 100 μM), cytotoxicity assessed by lactate dehydrogenase release to the media increased dose-dependently. LLC-PK1 cells produced hydrogen peroxide, visualized by 2,7-dichlorodihydrofluorescein assay by the treatment with 100 μM CsA, that was blocked by the treatment with catalase. The cytotoxicity of CsA significantly decreased either by the treatment with catalase, mannitol, or deferoxamine, but not with superoxide dismutase. These results suggest the role of hydrogen peroxide as the source of hydroxyl radical, which mainly contributes to CsA-induced LLC-PK1 injury.http://www.sciencedirect.com/science/article/pii/S1347861319327410
collection DOAJ
language English
format Article
sources DOAJ
author Masashi Nishida
Hiroshi Ogawa
Megumi Tamai
Kohhei Ishiwari
Kenji Hamaoka
spellingShingle Masashi Nishida
Hiroshi Ogawa
Megumi Tamai
Kohhei Ishiwari
Kenji Hamaoka
Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury
Journal of Pharmacological Sciences
author_facet Masashi Nishida
Hiroshi Ogawa
Megumi Tamai
Kohhei Ishiwari
Kenji Hamaoka
author_sort Masashi Nishida
title Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury
title_short Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury
title_full Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury
title_fullStr Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury
title_full_unstemmed Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury
title_sort role of hydrogen peroxide in cyclosporine-induced renal tubular cell (llc-pk1) injury
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2003-01-01
description We examined the role of hydrogen peroxide production in cyclosporine A (CsA)-induced LLC-PK1 injury. After exposure to CsA (0.1 μM – 100 μM), cytotoxicity assessed by lactate dehydrogenase release to the media increased dose-dependently. LLC-PK1 cells produced hydrogen peroxide, visualized by 2,7-dichlorodihydrofluorescein assay by the treatment with 100 μM CsA, that was blocked by the treatment with catalase. The cytotoxicity of CsA significantly decreased either by the treatment with catalase, mannitol, or deferoxamine, but not with superoxide dismutase. These results suggest the role of hydrogen peroxide as the source of hydroxyl radical, which mainly contributes to CsA-induced LLC-PK1 injury.
url http://www.sciencedirect.com/science/article/pii/S1347861319327410
work_keys_str_mv AT masashinishida roleofhydrogenperoxideincyclosporineinducedrenaltubularcellllcpk1injury
AT hiroshiogawa roleofhydrogenperoxideincyclosporineinducedrenaltubularcellllcpk1injury
AT megumitamai roleofhydrogenperoxideincyclosporineinducedrenaltubularcellllcpk1injury
AT kohheiishiwari roleofhydrogenperoxideincyclosporineinducedrenaltubularcellllcpk1injury
AT kenjihamaoka roleofhydrogenperoxideincyclosporineinducedrenaltubularcellllcpk1injury
_version_ 1725174165625896960

Similar Items