Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury

Role of Hydrogen Peroxide in Cyclosporine-Induced Renal Tubular Cell (LLC-PK1) Injury

We examined the role of hydrogen peroxide production in cyclosporine A (CsA)-induced LLC-PK1 injury. After exposure to CsA (0.1 μM – 100 μM), cytotoxicity assessed by lactate dehydrogenase release to the media increased dose-dependently. LLC-PK1 cells produced hydrogen peroxide, visualized by 2,7-di...

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Bibliographic Details
Main Authors: Masashi Nishida, Hiroshi Ogawa, Megumi Tamai, Kohhei Ishiwari, Kenji Hamaoka
Format: Article
Language:English
Published: Elsevier 2003-01-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319327410
Description
Summary:We examined the role of hydrogen peroxide production in cyclosporine A (CsA)-induced LLC-PK1 injury. After exposure to CsA (0.1 μM – 100 μM), cytotoxicity assessed by lactate dehydrogenase release to the media increased dose-dependently. LLC-PK1 cells produced hydrogen peroxide, visualized by 2,7-dichlorodihydrofluorescein assay by the treatment with 100 μM CsA, that was blocked by the treatment with catalase. The cytotoxicity of CsA significantly decreased either by the treatment with catalase, mannitol, or deferoxamine, but not with superoxide dismutase. These results suggest the role of hydrogen peroxide as the source of hydroxyl radical, which mainly contributes to CsA-induced LLC-PK1 injury.
ISSN:1347-8613

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