Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1

Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1

Summary: Precise measurement of action potentials (APs) is needed to observe electrical activity and cellular communication within cardiac tissue. Voltage-sensitive dyes (VSDs) are traditionally used to measure cardiac APs; however, they require acute chemical addition that prevents chronic imaging....

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Main Authors: Sanaya N. Shroff, Shoshana L. Das, Hua-an Tseng, Jad Noueihed, Fernando Fernandez, John A. White, Christopher S. Chen, Xue Han
Format: Article
Language:English
Published: Elsevier 2020-04-01
Series:iScience
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004220301589
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spelling doaj-80eb3f956de6450181eedcf9ffb18acc2020-11-25T02:44:17ZengElsevieriScience2589-00422020-04-01234Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1Sanaya N. Shroff0Shoshana L. Das1Hua-an Tseng2Jad Noueihed3Fernando Fernandez4John A. White5Christopher S. Chen6Xue Han7Department of Biomedical Engineering, Boston University, Boston, MA 02215, USADepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USA; Harvard-MIT Program in Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USADepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USADepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USADepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USADepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USADepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA; Corresponding authorDepartment of Biomedical Engineering, Boston University, Boston, MA 02215, USA; Corresponding authorSummary: Precise measurement of action potentials (APs) is needed to observe electrical activity and cellular communication within cardiac tissue. Voltage-sensitive dyes (VSDs) are traditionally used to measure cardiac APs; however, they require acute chemical addition that prevents chronic imaging. Genetically encoded voltage indicators (GEVIs) enable long-term studies of APs without the need of chemical additions, but current GEVIs used in cardiac tissue exhibit poor kinetics and/or low signal to noise (SNR). Here, we demonstrate the use of Archon1, a recently developed GEVI, in hiPSC-derived cardiomyocytes (CMs). When expressed in CMs, Archon1 demonstrated fast kinetics comparable with patch-clamp electrophysiology and high SNR significantly greater than the VSD Di-8-ANEPPS. Additionally, Archon1 enabled monitoring of APs across multiple cells simultaneously in 3D cardiac tissues. These results highlight Archon1's capability to investigate the electrical activity of CMs in a variety of applications and its potential to probe functionally complex in vitro models, as well as in vivo systems. : Biotechnology; Bioelectronics; Technical Aspects of Cell Biology; Electronic Materials Subject Areas: Biotechnology, Bioelectronics, Technical Aspects of Cell Biology, Electronic Materialshttp://www.sciencedirect.com/science/article/pii/S2589004220301589
collection DOAJ
language English
format Article
sources DOAJ
author Sanaya N. Shroff
Shoshana L. Das
Hua-an Tseng
Jad Noueihed
Fernando Fernandez
John A. White
Christopher S. Chen
Xue Han
spellingShingle Sanaya N. Shroff
Shoshana L. Das
Hua-an Tseng
Jad Noueihed
Fernando Fernandez
John A. White
Christopher S. Chen
Xue Han
Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1
iScience
author_facet Sanaya N. Shroff
Shoshana L. Das
Hua-an Tseng
Jad Noueihed
Fernando Fernandez
John A. White
Christopher S. Chen
Xue Han
author_sort Sanaya N. Shroff
title Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1
title_short Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1
title_full Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1
title_fullStr Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1
title_full_unstemmed Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1
title_sort voltage imaging of cardiac cells and tissue using the genetically encoded voltage sensor archon1
publisher Elsevier
series iScience
issn 2589-0042
publishDate 2020-04-01
description Summary: Precise measurement of action potentials (APs) is needed to observe electrical activity and cellular communication within cardiac tissue. Voltage-sensitive dyes (VSDs) are traditionally used to measure cardiac APs; however, they require acute chemical addition that prevents chronic imaging. Genetically encoded voltage indicators (GEVIs) enable long-term studies of APs without the need of chemical additions, but current GEVIs used in cardiac tissue exhibit poor kinetics and/or low signal to noise (SNR). Here, we demonstrate the use of Archon1, a recently developed GEVI, in hiPSC-derived cardiomyocytes (CMs). When expressed in CMs, Archon1 demonstrated fast kinetics comparable with patch-clamp electrophysiology and high SNR significantly greater than the VSD Di-8-ANEPPS. Additionally, Archon1 enabled monitoring of APs across multiple cells simultaneously in 3D cardiac tissues. These results highlight Archon1's capability to investigate the electrical activity of CMs in a variety of applications and its potential to probe functionally complex in vitro models, as well as in vivo systems. : Biotechnology; Bioelectronics; Technical Aspects of Cell Biology; Electronic Materials Subject Areas: Biotechnology, Bioelectronics, Technical Aspects of Cell Biology, Electronic Materials
url http://www.sciencedirect.com/science/article/pii/S2589004220301589
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