Extraction of Benzo[a]pyrene from Moist Snuff with Water or Artificial Saliva (Part 2)

• Main Authors: Moldoveanu Serban C., Harrison Andrew
• Format: Article
• Language: English
• Published: Sciendo 2020-05-01
• Series: Beiträge zur Tabakforschung International
• Subjects: moist snuff; benzo[a]pyrene; bap; extractability; artificial saliva
• Online Access: https://doi.org/10.2478/cttr-2020-0003

The present study evaluated the in vitro extraction of benzo[a]pyrene (BaP) from moist snuff into water and into artificial saliva. A similar, previous study evaluated the levels of BaP that remained in the moist snuff after the extraction but did not measure the levels of BaP in the water or saliva extract. The previous study showed that the remaining levels of BaP in the solid material were between 96.3% and 109.6% relative to the initial level of BaP, when the snuff was washed with water and between 99.4% and 108.3% from the initial level of BaP, when the snuff was washed with both saliva and water. Nine moist snuff samples (eight being the same brands as evaluated in the previous study) were analyzed in the present study. Several improvements were made compared to the previous study regarding the extraction conditions. The extraction was performed for 1 h at 37 °C, using a mechanical agitator.