Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus

Abstract Background Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently caus...

Full description

Bibliographic Details
Main Authors: Cleide Mara Rosa da Silva, Rosa Maria Chura-Chambi, Lennon Ramos Pereira, Yraima Cordeiro, Luís Carlos de Souza Ferreira, Ligia Morganti
Format: Article
Language:English
Published: BMC 2018-12-01
Series:BMC Biotechnology
Subjects:
Protein refolding
Inclusion bodies
NS1
Dengue virus and Zika virus
High hydrostatic pressure
Alkaline pH
Online Access:http://link.springer.com/article/10.1186/s12896-018-0486-2

Internet

http://link.springer.com/article/10.1186/s12896-018-0486-2

Similar Items