Ultrastructural characteristics of the mitochondria in experimental myocardial ischemia

• Main Authors: Loskutov O.A., Pivovarova O.A.
• Format: Article
• Language: Russian
• Published: Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy 2013-01-01
• Series: Морфологія
• Subjects: experimental diabetes mellitus; mitochondria; preconditioning
• Online Access: http://www.morphology.dp.ua/_pub/MORPHO-2013-07-01/13loaeim.pdf

In this investigation, on the model of isolated rat heart mitochondria studied ultrastructural reorganization and established prekondetsioniruyuschee action of sevoflurane. The study involved 47 white rats of Wistar, with a mass of 234,00±2,64 g, at the age of 5-6 months. Group I consisted of 20 individuals with artificial chemical diabetes treated with glybenclamid. Group II (27 individuals) was a control group. Ischemia was modeled after 30-minutes of sevofluran anesthesia and composed 20,4±0,30 minutes in both groups. As a result of the research it has been noted that the percent of structurally altered mitochondria in subsarcolemmal and intramiofibrill subpopulations in group ІІ was accordingly on 60,6±2,1% and on 67,1±3,4% less, in relation to the changes in group I. At the same time, the average diameter of the mitochondria in animals of group II was lower than those values observed in the first group, both in subsarcolemmal and in intramiofibrill subpopulations, accordingly 21,65±1,3% and 28,57±1,12%. Thus, the cardioprotective effect of sevoflurane, is a powerful mechanism of anti-ischemic action, the point of application of which are both of mitochondrial subsarcolemmal subpopulation and intramiofibrillyar mitochondrial subpopulation.