The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Kurdistan region, Iraq

• Main Author: D.A. Al-Mashhadany
• Format: Article
• Language: Arabic
• Published: University of Mosul, College of Veterinary Medicine 2019-09-01
• Series: Iraqi Journal of Veterinary Sciences
• Subjects: brucella antibodies; milk; mrt sensitivity; brucella abortus; brucella melitensis
• Online Access: https://vetmedmosul.com/article_163085_d9ca4f553829974fae204429e79c407a.pdf

This study was undertaken to monitor <em>Brucella</em> antibodies in the milk of cows and buffaloes in Erbil Governorate, Kurdistan Region, Iraq, using mik ring test (MRT) assay. A total of 210 samples of milk (130 from cows and 80 from buffaloes) were randomly collected from lactating females. The overall prevalence of <em>Brucella</em> antibodies in all the milk samples was 8.6% (18/210). The highest rate was 9.2% found in the cow milk (12/130), while the lowest rate was 7.5% of the buffalo’s milk (6/80). Out of 210 milk samples, only 15 (7.1%) were culture-positive for <em>Brucella</em>; about 7.7% (10/130) and 6.3% (5/80) from cows and buffaloes respectively. In terms of comparison between MRT and standard milk culture method, MRT was found more sensitive (83%), specific (98%), with the accuracy of 97% in comparison to the employed culture approach to detect Brucellosis agents in milk. The results also revealed that 70% and 60% of isolates were <em>Brucella abortus</em>, while 30% and 40% were <em>Brucella melitensis</em> from the milk of cow and buffaloes respectively. The highest rate of frequency for <em>Brucella</em> antibodies according to MRT was found in February (12.1%), while the lowest rate was found in June (5.7%). This study emphasizes that Brucellosis is still a significant public health hazard in the Kurdistan region. The study recommends MRT adoption in routine monitoring of brucellosis in milk collection centre, dairy factories, and farm. Consumers are also recommended to sufficiently heat the milk to destroy this foodborne pathogen before consumption or industrial processing.