Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution

Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution

Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic pept...

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Main Authors: Irina Boggs, Brad Hine, Grant Smolenski, Kasper Hettinga, Lina Zhang, Thomas T. Wheeler
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916303018
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spelling doaj-8096fa93d5c24068891cd65bfd22aada2020-11-25T02:45:42ZengElsevierData in Brief2352-34092016-09-0185255Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involutionIrina Boggs0Brad Hine1Grant Smolenski2Kasper Hettinga3Lina Zhang4Thomas T. Wheeler5Dairy Foods, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New ZealandCSIRO, Agriculture Flagship, Chiswick, Armidale, NSW 2350, AustraliaDairy Foods, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New ZealandWageningen University, Dairy Science and Technology, FQD Group, PO Box 17, 9, 6700AA Wageningen, The NetherlandsWageningen University, Dairy Science and Technology, FQD Group, PO Box 17, 9, 6700AA Wageningen, The NetherlandsDairy Foods, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand; Corresponding author. Tel.: +64 7 838 5196.Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication “Changes in the repertoire of bovine milk proteins during mammary involution” (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011. Keywords: Host defence, Dairy, Drying off, Proteomicshttp://www.sciencedirect.com/science/article/pii/S2352340916303018
collection DOAJ
language English
format Article
sources DOAJ
author Irina Boggs
Brad Hine
Grant Smolenski
Kasper Hettinga
Lina Zhang
Thomas T. Wheeler
spellingShingle Irina Boggs
Brad Hine
Grant Smolenski
Kasper Hettinga
Lina Zhang
Thomas T. Wheeler
Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
Data in Brief
author_facet Irina Boggs
Brad Hine
Grant Smolenski
Kasper Hettinga
Lina Zhang
Thomas T. Wheeler
author_sort Irina Boggs
title Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
title_short Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
title_full Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
title_fullStr Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
title_full_unstemmed Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
title_sort proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-09-01
description Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication “Changes in the repertoire of bovine milk proteins during mammary involution” (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011. Keywords: Host defence, Dairy, Drying off, Proteomics
url http://www.sciencedirect.com/science/article/pii/S2352340916303018
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AT grantsmolenski proteomicsdatainsupportofthequantificationofthechangesofbovinemilkproteinsduringmammaryglandinvolution
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