Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production

Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production

The cellulolytic bacterium Clostridium thermocellum is a promising candidate for lignocellulosic biofuel production; however, ethanol titer needs to be improved for commercialization. To understand the factors limiting ethanol titer in C. thermocellum, we developed a cell-free extract reaction (CFER...

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Bibliographic Details
Main Authors: Jingxuan Cui, David Stevenson, Travis Korosh, Daniel Amador-Noguez, Daniel G. Olson, Lee R. Lynd
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Energy Research
Subjects:
biofuel
cellulose
alcohol dehydrogenase (Adh)
quenching
metabolomics
pyruvate kinase (PYK)
Online Access:https://www.frontiersin.org/article/10.3389/fenrg.2020.00072/full

Internet

https://www.frontiersin.org/article/10.3389/fenrg.2020.00072/full

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