In vitro application of integrated selection index for screening drought tolerant genotypes in common wheat

• Main Authors: Ezatollah FARSHADFAR, Reza Amiri
• Format: Article
• Language: English
• Published: University of Ljubljana, Biotechnical Faculty 2016-10-01
• Series: Acta Agriculturae Slovenica
• Subjects: biplot analysis, mature embryo culture, drought stress, physiological indicators, principal component analysis, Triticum aestivum
• Online Access: http://ojs.aas.bf.uni-lj.si/index.php/AAS/article/view/127

<p style="margin: 0cm -0.05pt 0pt 0cm; text-align: justify; line-height: normal; tab-stops: 453.55pt;"><span style="font-size: 9pt;" lang="EN-GB"><span style="font-family: Times New Roman;">This experiment was conducted on 20 wheat genotypes during 2010-2011 growing season at the Razi University, Kermanshah, Iran. A completely randomized design with six replications was used for callus induction and a 20 × 2 factorial experiment with three replications was used for response of genotypes to <em>in vitro</em> drought stress. ANOVA exhibited highly significant differences among the genotypes for callus growth rate, relative fresh mass growth, relative growth rate, callus water content, percent of callus chlorosis and proline content under stress condition (15 % PEG). PCA showed that the integrated selection index was correlated with callus growth index, relative fresh mass growth, relative growth rate and proline content indicating that these screening techniques can be useful for selecting drought tolerant genotypes. Screening drought tolerant genotypes and <em>in vitro</em> indicators of drought tolerance using mean rank, standard deviation of ranks and biplot analysis, discriminated genotypes 2, 18 and 10 as the most drought tolerant. Therefore they are recommended to be used as parents for genetic analysis, gene mapping and improvement of drought tolerance.</span></span></p>