Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process

In order to find an explanation for corticosteroid resistance we assessed whether inhibition by dexamethasone (DEX) of the stimulated production of TNF-∝, IL-6, PGE2 and LTB4 by peripheral blood mononuclear cells (MNC) depends on binding to the glucocorticoid receptor (GR), and whether it is determi...

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Main Authors:	G. S. Madretsma, A. P. M. van Dijk, C. J. A. M. Tak, J. H. P. Wilson, F. J. Zijlstra
Format:	Article
Language:	English
Published:	Hindawi Limited 1996-01-01
Series:	Mediators of Inflammation
Online Access:	http://dx.doi.org/10.1155/S0962935196000166

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spelling	doaj-8054adf4640a465b862f59d8aa883eb32020-11-24T23:29:53ZengHindawi LimitedMediators of Inflammation0962-93511466-18611996-01-015210010310.1155/S0962935196000166Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated processG. S. Madretsma0A. P. M. van Dijk1C. J. A. M. Tak2J. H. P. Wilson3F. J. Zijlstra4Department of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The NetherlandsDepartment of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The NetherlandsDepartment of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The NetherlandsDepartment of Internal Medicine II, University Hospital Dijkzigt, Rotterdam, The NetherlandsDepartment of Pharmacology, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The NetherlandsIn order to find an explanation for corticosteroid resistance we assessed whether inhibition by dexamethasone (DEX) of the stimulated production of TNF-∝, IL-6, PGE2 and LTB4 by peripheral blood mononuclear cells (MNC) depends on binding to the glucocorticoid receptor (GR), and whether it is determined by the number or the affinity of the GR of these cells. GR number and affinity of MNC were determined by means of a whole cell DEX binding assay. MNC were incubated with DEX and LPS or A23187 in the absence or presence of RU486, a potent steroid antagonist. DEX caused a concentration dependent inhibition of TNF-∝, IL-6 and PGE2 production but had no effect on LTB4 production. RU486 significantly blocked the effect of DEX, but no correlations were found between the inhibition of mediator release and the Kd or receptor number.http://dx.doi.org/10.1155/S0962935196000166
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	G. S. Madretsma A. P. M. van Dijk C. J. A. M. Tak J. H. P. Wilson F. J. Zijlstra
spellingShingle	G. S. Madretsma A. P. M. van Dijk C. J. A. M. Tak J. H. P. Wilson F. J. Zijlstra Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process Mediators of Inflammation
author_facet	G. S. Madretsma A. P. M. van Dijk C. J. A. M. Tak J. H. P. Wilson F. J. Zijlstra
author_sort	G. S. Madretsma
title	Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process
title_short	Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process
title_full	Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process
title_fullStr	Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process
title_full_unstemmed	Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process
title_sort	inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process
publisher	Hindawi Limited
series	Mediators of Inflammation
issn	0962-9351 1466-1861
publishDate	1996-01-01
description	In order to find an explanation for corticosteroid resistance we assessed whether inhibition by dexamethasone (DEX) of the stimulated production of TNF-∝, IL-6, PGE2 and LTB4 by peripheral blood mononuclear cells (MNC) depends on binding to the glucocorticoid receptor (GR), and whether it is determined by the number or the affinity of the GR of these cells. GR number and affinity of MNC were determined by means of a whole cell DEX binding assay. MNC were incubated with DEX and LPS or A23187 in the absence or presence of RU486, a potent steroid antagonist. DEX caused a concentration dependent inhibition of TNF-∝, IL-6 and PGE2 production but had no effect on LTB4 production. RU486 significantly blocked the effect of DEX, but no correlations were found between the inhibition of mediator release and the Kd or receptor number.
url	http://dx.doi.org/10.1155/S0962935196000166
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Inhibition of the production of mediators of inflammation by corticosteroids is a glucocorticoid receptor-mediated process

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