Kapang endofit dari Taman Nasional Gunung Halimun sebagai penghambat pertumbuhan mikroba patogen Salmonella thypi dan Candida albicans

• Main Authors: Ruth Ruth, Puspita Suci Wulandari
• Format: Article
• Language: English
• Published: Indonesian Biological Society 2012-02-01
• Series: Journal of Biological Researches
• Subjects: endophytic fungi; screening; antimicrobial compound; TLC
• Online Access: http://berkalahayati.org/journal/85454e82
• ISSN: 0852-6834; 2337-389X

Endophytic fungi live inside plants without harming the host. The purpose of this research was to screen endophytic fungi whichcould inhibit Salmonella thypi and Candida albicans, as well as characterizing of endophytic fungi, and antimicrobial compoundproduced by endophytic fungi. Some methods were used on this research. Diffusion agar plate methode was used to screen endophyticfungi which could produce antimicrobial compound against Salmonella thypi and Candida albicans. Standard plate count was used tomeasure fungi growth. Antimicrobial compound produced by endophytic fungi was analized by TLC and HPLC technique, compared withstandard antibiotic, chloramphenicol and nystatin. The result showed 5 kinds of endophytic fungi produced antimicrobial compoundsagainst Salmonella thypi. The largest zone of inhibition was 115 mm2 shown by Hl.25F.112. Two kinds of endophytic fungi were ableto inhibit Candida albicans with the largest inhibiting zone was 164 mm2 shown by Hl.108F.481. The morphology of Hl.108F.481indicated that this fungus had vertical hypha with sporangium at the end of the hypha and Hl.25F.112 had partioned hypha andoval-shape ascus. TLC and HPLC analysis showed that water extract of Hl.25F.112 (1s), chloroform extract of Hl.25F.112 (1c) andchloroform ectract of Hl.108F.481 (2c) contained anti-microbial compound with retention time (RT) closed to chloramphenicol andwater phase of Hl.25F.112 (1s) closed to nystatin.