A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection
In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. AT...
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MDPI AG
2018-09-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/10/3281 |
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doaj-801819a1710646a9981a30559fc29f092020-11-25T00:58:02ZengMDPI AGSensors1424-82202018-09-011810328110.3390/s18103281s18103281A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP DetectionJingjing Zhang0Chunzheng Yang1Chaoqun Niu2Chen Liu3Xuepin Cai4Jie Du5Yong Chen6State Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, College of Information Science & Technology, College of Materials and Chemical Engineering, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaIn this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2 = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106 to 2.988 × 106 M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive.http://www.mdpi.com/1424-8220/18/10/3281ATP detectionlabel-free fluorescenceenzyme-freegraphene oxidelogic gate |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Jingjing Zhang Chunzheng Yang Chaoqun Niu Chen Liu Xuepin Cai Jie Du Yong Chen |
| spellingShingle |
Jingjing Zhang Chunzheng Yang Chaoqun Niu Chen Liu Xuepin Cai Jie Du Yong Chen A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection Sensors ATP detection label-free fluorescence enzyme-free graphene oxide logic gate |
| author_facet |
Jingjing Zhang Chunzheng Yang Chaoqun Niu Chen Liu Xuepin Cai Jie Du Yong Chen |
| author_sort |
Jingjing Zhang |
| title |
A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
| title_short |
A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
| title_full |
A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
| title_fullStr |
A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
| title_full_unstemmed |
A Label-Free Fluorescent AND Logic Gate Aptasensor for Sensitive ATP Detection |
| title_sort |
label-free fluorescent and logic gate aptasensor for sensitive atp detection |
| publisher |
MDPI AG |
| series |
Sensors |
| issn |
1424-8220 |
| publishDate |
2018-09-01 |
| description |
In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2 = 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106 to 2.988 × 106 M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive. |
| topic |
ATP detection label-free fluorescence enzyme-free graphene oxide logic gate |
| url |
http://www.mdpi.com/1424-8220/18/10/3281 |
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