Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants

The in vitro synthesis of phytate was studied in common bean fruit explants. Different concentrations of sucrose; phosphorus (P); myo-inositol; abscisic acid (ABA); glutamine and methionine, were tested. Fixed concentrations of these compounds were tested at different periods (0, 3, 6 and 9 days). V...

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Main Authors: Cileide Maria Medeiros Coelho, Cláudia Mattos Bellato, Andréia Karime Marcelino Garcia, Victor Alexandre Vitorello, Ricardo Antunes Azevedo
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2008-02-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100020
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spelling doaj-8013af4dbaee4df89452ba53e6ff14dc2020-11-24T23:30:05ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242008-02-0151116317310.1590/S1516-89132008000100020Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explantsCileide Maria Medeiros CoelhoCláudia Mattos BellatoAndréia Karime Marcelino GarciaVictor Alexandre VitorelloRicardo Antunes AzevedoThe in vitro synthesis of phytate was studied in common bean fruit explants. Different concentrations of sucrose; phosphorus (P); myo-inositol; abscisic acid (ABA); glutamine and methionine, were tested. Fixed concentrations of these compounds were tested at different periods (0, 3, 6 and 9 days). Variation in phytate coincided with different concentrations of sucrose, myo-inositol, P and ABA for the duration tested. These compounds caused an accumulation of phytate and were more effective in the presence of myo-inositol and P. The accumulation of P varied less than phytate for the different treatments tested in vitro. In conclusion, P, sucrose, ABA, and myo-inositol caused an increase in the phytate of bean seed, showing that it could be possible to alter its content by culturing bean fruit explants in vitro.<br>O fósforo é armazenado na forma de fitato nas sementes, o qual forma complexos estáveis e insolúveis com minerais e proteínas, conferindo efeito antinutriente. A síntese de fitato foi estudada em cultivo de explantes de fruto de feijão in vitro sob diferentes concentrações de sacarose, fósforo (P), mio-inositol, ácido abscísico (ABA), glutamina e metionina. Fixada a concentração destes compostos, testou-se os diferentes tempos de cultivo (0, 3, 6 e 9 dias). A variação no acúmulo de fitato ocorreu na presença de sacarose, mio-inositol, P e ABA nas diferentes concentrações e tempos testados. O acúmulo mais efetivo de fitato ocorreu na presença de mio-inositol e P. O acúmulo de P variou menos do que fitato em todos os tratamentos. Em conclusão, P, sacarose, ABA e mio-inositol causaram aumento no fitato acumulado nas sementes, mostrando que foi possível alterar a síntese de fitato em cultivo de explantes de fruto de feijão.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100020Abscisic acidglutaminephosphorusphytic acidsucrose
collection DOAJ
language English
format Article
sources DOAJ
author Cileide Maria Medeiros Coelho
Cláudia Mattos Bellato
Andréia Karime Marcelino Garcia
Victor Alexandre Vitorello
Ricardo Antunes Azevedo
spellingShingle Cileide Maria Medeiros Coelho
Cláudia Mattos Bellato
Andréia Karime Marcelino Garcia
Victor Alexandre Vitorello
Ricardo Antunes Azevedo
Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants
Brazilian Archives of Biology and Technology
Abscisic acid
glutamine
phosphorus
phytic acid
sucrose
author_facet Cileide Maria Medeiros Coelho
Cláudia Mattos Bellato
Andréia Karime Marcelino Garcia
Victor Alexandre Vitorello
Ricardo Antunes Azevedo
author_sort Cileide Maria Medeiros Coelho
title Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants
title_short Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants
title_full Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants
title_fullStr Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants
title_full_unstemmed Variation in phytate accumulation in common bean (Phaseolus vulgaris L.) fruit explants
title_sort variation in phytate accumulation in common bean (phaseolus vulgaris l.) fruit explants
publisher Instituto de Tecnologia do Paraná (Tecpar)
series Brazilian Archives of Biology and Technology
issn 1516-8913
1678-4324
publishDate 2008-02-01
description The in vitro synthesis of phytate was studied in common bean fruit explants. Different concentrations of sucrose; phosphorus (P); myo-inositol; abscisic acid (ABA); glutamine and methionine, were tested. Fixed concentrations of these compounds were tested at different periods (0, 3, 6 and 9 days). Variation in phytate coincided with different concentrations of sucrose, myo-inositol, P and ABA for the duration tested. These compounds caused an accumulation of phytate and were more effective in the presence of myo-inositol and P. The accumulation of P varied less than phytate for the different treatments tested in vitro. In conclusion, P, sucrose, ABA, and myo-inositol caused an increase in the phytate of bean seed, showing that it could be possible to alter its content by culturing bean fruit explants in vitro.<br>O fósforo é armazenado na forma de fitato nas sementes, o qual forma complexos estáveis e insolúveis com minerais e proteínas, conferindo efeito antinutriente. A síntese de fitato foi estudada em cultivo de explantes de fruto de feijão in vitro sob diferentes concentrações de sacarose, fósforo (P), mio-inositol, ácido abscísico (ABA), glutamina e metionina. Fixada a concentração destes compostos, testou-se os diferentes tempos de cultivo (0, 3, 6 e 9 dias). A variação no acúmulo de fitato ocorreu na presença de sacarose, mio-inositol, P e ABA nas diferentes concentrações e tempos testados. O acúmulo mais efetivo de fitato ocorreu na presença de mio-inositol e P. O acúmulo de P variou menos do que fitato em todos os tratamentos. Em conclusão, P, sacarose, ABA e mio-inositol causaram aumento no fitato acumulado nas sementes, mostrando que foi possível alterar a síntese de fitato em cultivo de explantes de fruto de feijão.
topic Abscisic acid
glutamine
phosphorus
phytic acid
sucrose
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100020
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