The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats

Abstract Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. Material and Methods Rattus norvegicus (n=75) were divided int...

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Main Authors: Isadora Mello Vilarinho SOARES, Gustavo Vicentis de Oliveira FERNANDES, CAVALCANTE Larissa Cordeiro, Yulla Klinger Pereira de Carvalho LEITE, Dayseanny de Oliveira BEZERRA, Maria Acelina Martins de CARVALHO, Carmen Milena Rodrigues Siqueira CARVALHO
Format: Article
Language:English
Published: University of São Paulo 2019-04-01
Series:Journal of Applied Oral Science
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100440&lng=en&tlng=en
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spelling doaj-8009973cb1da46ebb6d386dd4d5435912020-11-25T02:17:46ZengUniversity of São PauloJournal of Applied Oral Science1678-77652019-04-0127010.1590/1678-7757-2018-0103S1678-77572019000100440The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in ratsIsadora Mello Vilarinho SOARESGustavo Vicentis de Oliveira FERNANDESCAVALCANTE Larissa CordeiroYulla Klinger Pereira de Carvalho LEITEDayseanny de Oliveira BEZERRAMaria Acelina Martins de CARVALHOCarmen Milena Rodrigues Siqueira CARVALHOAbstract Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn’s post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon® (p<0.05). No statistically significant difference was found between the groups regarding bone formation at the three experimental points in time. Osteopontin expression corroborated the intensity of bone formation. Fluorescence microscopy revealed positive labeling with Q-Tracker® in hDPSCs before transplantation and tissue repair. Conclusion The results suggest that the combination of Hemospon®, Aloe vera and hDPSCs is a form of clinical treatment for the repair of non-critical bone defects that reduces the inflammatory cascade’s effects.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100440&lng=en&tlng=enBone regenerationStem cellsStem cell transplantationOsteopontinHerbal medicines
collection DOAJ
language English
format Article
sources DOAJ
author Isadora Mello Vilarinho SOARES
Gustavo Vicentis de Oliveira FERNANDES
CAVALCANTE Larissa Cordeiro
Yulla Klinger Pereira de Carvalho LEITE
Dayseanny de Oliveira BEZERRA
Maria Acelina Martins de CARVALHO
Carmen Milena Rodrigues Siqueira CARVALHO
spellingShingle Isadora Mello Vilarinho SOARES
Gustavo Vicentis de Oliveira FERNANDES
CAVALCANTE Larissa Cordeiro
Yulla Klinger Pereira de Carvalho LEITE
Dayseanny de Oliveira BEZERRA
Maria Acelina Martins de CARVALHO
Carmen Milena Rodrigues Siqueira CARVALHO
The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
Journal of Applied Oral Science
Bone regeneration
Stem cells
Stem cell transplantation
Osteopontin
Herbal medicines
author_facet Isadora Mello Vilarinho SOARES
Gustavo Vicentis de Oliveira FERNANDES
CAVALCANTE Larissa Cordeiro
Yulla Klinger Pereira de Carvalho LEITE
Dayseanny de Oliveira BEZERRA
Maria Acelina Martins de CARVALHO
Carmen Milena Rodrigues Siqueira CARVALHO
author_sort Isadora Mello Vilarinho SOARES
title The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
title_short The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
title_full The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
title_fullStr The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
title_full_unstemmed The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
title_sort influence of aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats
publisher University of São Paulo
series Journal of Applied Oral Science
issn 1678-7765
publishDate 2019-04-01
description Abstract Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn’s post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon® (p<0.05). No statistically significant difference was found between the groups regarding bone formation at the three experimental points in time. Osteopontin expression corroborated the intensity of bone formation. Fluorescence microscopy revealed positive labeling with Q-Tracker® in hDPSCs before transplantation and tissue repair. Conclusion The results suggest that the combination of Hemospon®, Aloe vera and hDPSCs is a form of clinical treatment for the repair of non-critical bone defects that reduces the inflammatory cascade’s effects.
topic Bone regeneration
Stem cells
Stem cell transplantation
Osteopontin
Herbal medicines
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100440&lng=en&tlng=en
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