Histopathologic Evaluation of Radio-Protective Effect of Hesperidin on the Liver of Sprague Dawely Rats

• Main Authors: Zh Ghorbani, R Fardid, G H Haddadi, A Derakhshanfar, A Kouhpayeh, Z Haddadi
• Format: Article
• Language: English
• Published: Shiraz University of Medical Sciences 2020-02-01
• Series: Journal of Biomedical Physics and Engineering
• Subjects: gamma rays; hesperidin; radio-protector; hyperemia; capillarization
• Online Access: http://jbpe.sums.ac.ir/article_45724_792cf52388a1cc484cfaed88bb001bcc.pdf

Background: Hesperidin is a bioflavonoid glycoside mainly found in citrus fruit and has been shown radio-protective potential in various measurement systems. <br />Objective: In this article aims to investigate the radio-protective effect of hesperidin on the liver of Sprague Dawely rats. <br />Material and Methods: In this clinical study, 40 male rats were selected randomly and divided into 8 groups. Group 1 did not receive radiation and hesperidin (sham control). Group 2 received only 100 mg/kg body weight (b.w) of hesperidin for 7 consecutive days (HES group); group 3 exposed to dose of 2Gy whole body gamma radiation (2Gy group), and group 4 and 5 received 50 and 100 mg/kg b.w of HES for 7 consecutive days before 2 Gy gamma radiation, respectively.Group 6 exposed to dose of 8Gy gamma radiation (8Gy group); group 7 and 8 received 50 and 100 mg/kg b.w of HES for 7 days before 8Gy gamma irradiation, respectively. Histopathological evaluation was perfomred 24 hours after radiation. <br />Results: Administration of hesperidin (50 mg/kg b.w, 7 days) before 2Gy of gamma irradiation led to remove inflammatory mononuclear cells in the portal space. Microscopic findings in the groups receiving two doses of hesperidin (50 and 100 mg/kg b.w, orally, 7 days), before 8Gy of gamma radiation, were similar in a way that extreme dilation of central veins to be seen, however, there was no capillarization. <br />Conclusion: HES can be offered as a suitable radio-protector in radiotherapy patients and radiation workers.