Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters
<p>Abstract</p> <p>Background</p> <p>Since it was first described in the mid-1990s, quantitative real time PCR (Q-PCR) has been widely used in many fields of biomedical research and molecular diagnostics. This method is routinely used to validate whole transcriptome ana...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-05-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/9/234 |