Comparison of Three RT-PCR Based Methods for Relative Quantification of mRNA

Comparison of Three RT-PCR Based Methods for Relative Quantification of mRNA

Comparison of three RT-PCR based methods: semi-quantitative, competitive and real--time RT-PCR for relative quantification of mRNA is presented. Aminopeptidase N expressed on human promyeloid HL-60 cell line, at basal and activated state, served as a model for comparison. HL-60 cells were stimulated...

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Bibliographic Details
Main Authors: Andreja Ambriović-Ristov, Sanja Kapitanović, Tamara Čačev, Jelka Gabrilovac, Davorka Breljak
Format: Article
Language:English
Published: University of Zagreb 2005-01-01
Series:Food Technology and Biotechnology
Subjects:
aminopeptidase N
APN
HL-60
RT-PCR
real-time PCR
competitive PCR
Online Access:http://hrcak.srce.hr/file/162952

Internet

http://hrcak.srce.hr/file/162952

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