Evaluation of the blood coagulation function, degree of inflammation as well as apoptosis in brain tissue of rat models with heat stroke

• Main Authors: Lin Zhao, Jun Li
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2017-03-01
• Series: Journal of Hainan Medical University
• Subjects: Heat stroke; Blood coagulation function; Inflammation; Brain injury; Apoptosis
• Online Access: http://www.hnykdxxb.com/PDF/201703/5.pdf

Objective: To study the blood coagulation function, degree of inflammation as well as apoptosis in brain tissue of rat models with heat stroke. Methods: Healthy male SD rats were selected as experimental animals and randomly divided into heat stroke group and control group, heat stroke group were raised under the condition of environment temperature (40.0±0.5) ℃ and relative humidity 60% to establish heat stroke models, the anus temperature reached 42.5 ℃, and the control group were conventionally raised; 60 and 120 min after model establishment, the blood coagulation function, serum inflammatory factor levels as well as brain water content, the number of apoptotic cells and apoptotic molecule expression were determined respectively. Results: (1) blood biochemical indexes: Activated partial thromboplastin time (APTT) and prothrombin time (PT) of heat stroke group were longer than those of control group, and serum D-Dimer content (D-D), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 levels were significantly higher than those of control group (P<0.05); (2) the brain tissue damage indexes: brain water content, the number of apoptotic cells, Caspase-3 expression and LC3-II/LC3-I ratio of heat stroke group were significantly higher than those of control group (P<0.05). Conclusions: There are blood coagulation dysfunction and systemic inflammation activation in heat stroke model rats, and the apoptosis in brain tissue increases obviously.