| Summary: | Objective: To study the blood coagulation function, degree of inflammation as well as
apoptosis in brain tissue of rat models with heat stroke. Methods: Healthy male SD rats
were selected as experimental animals and randomly divided into heat stroke group and
control group, heat stroke group were raised under the condition of environment temperature
(40.0±0.5) ℃ and relative humidity 60% to establish heat stroke models, the anus temperature
reached 42.5 ℃, and the control group were conventionally raised; 60 and 120 min after
model establishment, the blood coagulation function, serum inflammatory factor levels as
well as brain water content, the number of apoptotic cells and apoptotic molecule expression
were determined respectively. Results: (1) blood biochemical indexes: Activated partial
thromboplastin time (APTT) and prothrombin time (PT) of heat stroke group were longer than
those of control group, and serum D-Dimer content (D-D), tumor necrosis factor-α (TNF-α),
interleukin-1β (IL-1β) and IL-6 levels were significantly higher than those of control group
(P<0.05); (2) the brain tissue damage indexes: brain water content, the number of apoptotic
cells, Caspase-3 expression and LC3-II/LC3-I ratio of heat stroke group were significantly
higher than those of control group (P<0.05). Conclusions: There are blood coagulation
dysfunction and systemic inflammation activation in heat stroke model rats, and the apoptosis
in brain tissue increases obviously.
|
|---|
