Carvacrol Loaded Solid Lipid Nanoparticles of Propylene Glycol Monopalmitate and Glyceryl Monostearate: Preparation, Characterization, and Synergistic Antimicrobial Activity

• Main Authors: Junbo He, Shuangshuang Huang, Xiaotao Sun, Lijuan Han, Chao Chang, Weinong Zhang, Qixin Zhong
• Format: Article
• Language: English
• Published: MDPI AG 2019-08-01
• Series: Nanomaterials
• Subjects: solid lipid nanoparticles; carvacrol; propylene glycol monopalmitate; synergistic antimicrobial activity
• Online Access: https://www.mdpi.com/2079-4991/9/8/1162

To develop solid lipid nanoparticles (SLNs) with stable lipid matrix structures for the delivery of bioactive compounds, a new class of SLNs was studied using propylene glycol monopalmitate (PGMP) and glyceryl monostearate (GMS) mixtures and carvacrol as a model lipophilic antimicrobial. Stable SLNs were fabricated at PGMP:GMS mass ratios of 2:1 and 1:1, and the carvacrol loading was up to 30% of lipids with &gt;98% encapsulation efficiency and absence of visual instability. Fluorescence spectra and release profiles indicated the carvacrol was successfully encapsulated and homogeneously distributed within the SLNs. SLNs fabricated with equal masses of PGMP and GMS had better stability of carvacrol during storage and higher sphericity than those with a ratio of 2:1 and were much more effective than free carvacrol against <i>Escherichia coli</i> O157:H7 and <i>Staphylococcus aureus</i>. These findings demonstrated the potential applications of the studied SLNs in delivering lipophilic bioactive compounds in food and other products.