Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics

Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics

Leukaemic stem cells drive acute myeloid leukaemia (AML) progression and relapse but they are incompletely characterized. Here, the authors combine single-cell transcriptomics and clonal tracking using nuclear and mitochondrial somatic variants to distinguish healthy, pre-leukaemic and leukaemic ste...

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Main Authors: Lars Velten, Benjamin A. Story, Pablo Hernández-Malmierca, Simon Raffel, Daniel R. Leonce, Jennifer Milbank, Malte Paulsen, Aykut Demir, Chelsea Szu-Tu, Robert Frömel, Christoph Lutz, Daniel Nowak, Johann-Christoph Jann, Caroline Pabst, Tobias Boch, Wolf-Karsten Hofmann, Carsten Müller-Tidow, Andreas Trumpp, Simon Haas, Lars M. Steinmetz
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21650-1
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spelling doaj-7f3244539bc54da0bc5ec3fba0f6edbe2021-03-11T11:33:33ZengNature Publishing GroupNature Communications2041-17232021-03-0112111310.1038/s41467-021-21650-1Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomicsLars Velten0Benjamin A. Story1Pablo Hernández-Malmierca2Simon Raffel3Daniel R. Leonce4Jennifer Milbank5Malte Paulsen6Aykut Demir7Chelsea Szu-Tu8Robert Frömel9Christoph Lutz10Daniel Nowak11Johann-Christoph Jann12Caroline Pabst13Tobias Boch14Wolf-Karsten Hofmann15Carsten Müller-Tidow16Andreas Trumpp17Simon Haas18Lars M. Steinmetz19Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and TechnologyEuropean Molecular Biology Laboratory (EMBL), Genome Biology UnitHeidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)European Molecular Biology Laboratory (EMBL), Genome Biology UnitEuropean Molecular Biology Laboratory (EMBL), Genome Biology UnitEuropean Molecular Biology Laboratory (EMBL), Flow Cytometry Core FacilityDepartment of Internal Medicine V, Hematology, Oncology and Rheumatology, University of HeidelbergCentre for Genomic Regulation (CRG), The Barcelona Institute of Science and TechnologyCentre for Genomic Regulation (CRG), The Barcelona Institute of Science and TechnologyDepartment of Internal Medicine V, Hematology, Oncology and Rheumatology, University of HeidelbergDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg UniversityDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg UniversityDepartment of Internal Medicine V, Hematology, Oncology and Rheumatology, University of HeidelbergSwiss Federal Institute of Technology (ETH) Zurich, Department of Biosystems Science and EngineeringDepartment of Hematology and Oncology, Medical Faculty Mannheim, Heidelberg UniversityDepartment of Internal Medicine V, Hematology, Oncology and Rheumatology, University of HeidelbergHeidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)European Molecular Biology Laboratory (EMBL), Genome Biology UnitLeukaemic stem cells drive acute myeloid leukaemia (AML) progression and relapse but they are incompletely characterized. Here, the authors combine single-cell transcriptomics and clonal tracking using nuclear and mitochondrial somatic variants to distinguish healthy, pre-leukaemic and leukaemic stem cells in AML.https://doi.org/10.1038/s41467-021-21650-1
collection DOAJ
language English
format Article
sources DOAJ
author Lars Velten
Benjamin A. Story
Pablo Hernández-Malmierca
Simon Raffel
Daniel R. Leonce
Jennifer Milbank
Malte Paulsen
Aykut Demir
Chelsea Szu-Tu
Robert Frömel
Christoph Lutz
Daniel Nowak
Johann-Christoph Jann
Caroline Pabst
Tobias Boch
Wolf-Karsten Hofmann
Carsten Müller-Tidow
Andreas Trumpp
Simon Haas
Lars M. Steinmetz
spellingShingle Lars Velten
Benjamin A. Story
Pablo Hernández-Malmierca
Simon Raffel
Daniel R. Leonce
Jennifer Milbank
Malte Paulsen
Aykut Demir
Chelsea Szu-Tu
Robert Frömel
Christoph Lutz
Daniel Nowak
Johann-Christoph Jann
Caroline Pabst
Tobias Boch
Wolf-Karsten Hofmann
Carsten Müller-Tidow
Andreas Trumpp
Simon Haas
Lars M. Steinmetz
Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
Nature Communications
author_facet Lars Velten
Benjamin A. Story
Pablo Hernández-Malmierca
Simon Raffel
Daniel R. Leonce
Jennifer Milbank
Malte Paulsen
Aykut Demir
Chelsea Szu-Tu
Robert Frömel
Christoph Lutz
Daniel Nowak
Johann-Christoph Jann
Caroline Pabst
Tobias Boch
Wolf-Karsten Hofmann
Carsten Müller-Tidow
Andreas Trumpp
Simon Haas
Lars M. Steinmetz
author_sort Lars Velten
title Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
title_short Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
title_full Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
title_fullStr Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
title_full_unstemmed Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
title_sort identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2021-03-01
description Leukaemic stem cells drive acute myeloid leukaemia (AML) progression and relapse but they are incompletely characterized. Here, the authors combine single-cell transcriptomics and clonal tracking using nuclear and mitochondrial somatic variants to distinguish healthy, pre-leukaemic and leukaemic stem cells in AML.
url https://doi.org/10.1038/s41467-021-21650-1
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