Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice

Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice

The aim of this study was to evaluate the effects of different kinds of oligosaccharides on cecal microbiota. These oligosaccharides were a fructo-oligosaccharide (FOS) formulation consisting of 95% FOS (FOS95), a galacto-oligosaccharide (GOS) formulation consisting of 90% GOS (GOS90), and an isomal...

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Main Authors: Linlin Wang, Mingluo Pan, Dongyao Li, Yiting Yin, Tian Jiang, Shuguang Fang, Gang Wang, Jianxin Zhao, Hao Zhang, Wei Chen
Format: Article
Language:English
Published: Elsevier 2017-11-01
Series:Journal of Functional Foods
Subjects:
Microbiota
Cecal contents
16S rRNA metagenomics
Oligosaccharides
Constipation
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464617305686
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spelling doaj-7f2ef753fd204333852024d5cf165c912021-04-30T07:11:52ZengElsevierJournal of Functional Foods1756-46462017-11-0138486496Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated miceLinlin Wang0Mingluo Pan1Dongyao Li2Yiting Yin3Tian Jiang4Shuguang Fang5Gang Wang6Jianxin Zhao7Hao Zhang8Wei Chen9State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR ChinaJiangsu Wecare Biotechnology Co., Ltd, Wujiang 215200, PR ChinaJiangsu Wecare Biotechnology Co., Ltd, Wujiang 215200, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China; Corresponding authors at: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China; Corresponding authors at: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China.State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China; International Joint Research Center for Probiotics & Gut Health, Jiangnan University, Wuxi 214122, PR China; Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, PR ChinaThe aim of this study was to evaluate the effects of different kinds of oligosaccharides on cecal microbiota. These oligosaccharides were a fructo-oligosaccharide (FOS) formulation consisting of 95% FOS (FOS95), a galacto-oligosaccharide (GOS) formulation consisting of 90% GOS (GOS90), and an isomalto-oligosaccharide (IMO) formulation consisting of 90% IMO (IMO90), at dosages of 0.8, 4, and 8 g/d/kg bw. FOS95, IMO90, and GOS90 were each gavaged to BALB/c mice for 14 days. Sequencing of 16S rDNA amplicons showed that the abundance of Actinobacteria and TM7 dramatically decreased in constipation group. Feeding constipated mice with FOS95, IMO90, and GOS90 resulted in decreased ratio of Firmicutes to Bacteroidetes, and increased abundances of Bifidobacterium, Bacteroides, Rikenella, Lactobacillus, and Clostridium and decreased abundances of Oscillospira, Coprococcus, and Dorea at the genus level. Overall, our results demonstrate that FOS95, IMO90, and GOS90 modulate the cecal microbiome in constipated mice.http://www.sciencedirect.com/science/article/pii/S1756464617305686MicrobiotaCecal contents16S rRNA metagenomicsOligosaccharidesConstipation
collection DOAJ
language English
format Article
sources DOAJ
author Linlin Wang
Mingluo Pan
Dongyao Li
Yiting Yin
Tian Jiang
Shuguang Fang
Gang Wang
Jianxin Zhao
Hao Zhang
Wei Chen
spellingShingle Linlin Wang
Mingluo Pan
Dongyao Li
Yiting Yin
Tian Jiang
Shuguang Fang
Gang Wang
Jianxin Zhao
Hao Zhang
Wei Chen
Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice
Journal of Functional Foods
Microbiota
Cecal contents
16S rRNA metagenomics
Oligosaccharides
Constipation
author_facet Linlin Wang
Mingluo Pan
Dongyao Li
Yiting Yin
Tian Jiang
Shuguang Fang
Gang Wang
Jianxin Zhao
Hao Zhang
Wei Chen
author_sort Linlin Wang
title Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice
title_short Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice
title_full Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice
title_fullStr Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice
title_full_unstemmed Metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice
title_sort metagenomic insights into the effects of oligosaccharides on the microbial composition of cecal contents in constipated mice
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2017-11-01
description The aim of this study was to evaluate the effects of different kinds of oligosaccharides on cecal microbiota. These oligosaccharides were a fructo-oligosaccharide (FOS) formulation consisting of 95% FOS (FOS95), a galacto-oligosaccharide (GOS) formulation consisting of 90% GOS (GOS90), and an isomalto-oligosaccharide (IMO) formulation consisting of 90% IMO (IMO90), at dosages of 0.8, 4, and 8 g/d/kg bw. FOS95, IMO90, and GOS90 were each gavaged to BALB/c mice for 14 days. Sequencing of 16S rDNA amplicons showed that the abundance of Actinobacteria and TM7 dramatically decreased in constipation group. Feeding constipated mice with FOS95, IMO90, and GOS90 resulted in decreased ratio of Firmicutes to Bacteroidetes, and increased abundances of Bifidobacterium, Bacteroides, Rikenella, Lactobacillus, and Clostridium and decreased abundances of Oscillospira, Coprococcus, and Dorea at the genus level. Overall, our results demonstrate that FOS95, IMO90, and GOS90 modulate the cecal microbiome in constipated mice.
topic Microbiota
Cecal contents
16S rRNA metagenomics
Oligosaccharides
Constipation
url http://www.sciencedirect.com/science/article/pii/S1756464617305686
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