Preparation of Ganglioside GM1 by Supercritical CO₂ Extraction and Immobilized Sialidase

• Main Authors: Li Ji, Zhonghui Qiao, Xin Zhang, Xiaolei Cheng, Weiyang Wang, Fan Zhang, Yifa Zhou, Ye Yuan
• Format: Article
• Language: English
• Published: MDPI AG 2019-10-01
• Series: Molecules
• Subjects: ganglioside; gm1; supercritical co₂ extraction; immobilization; sialidase; purification
• Online Access: https://www.mdpi.com/1420-3049/24/20/3732

Monosialotetrahexosylganglioside (GM1) has good activity on brain diseases and was developed to be a drug applied in clinics for neurological disorders and nerve injury. It is difficult to isolate GM1 in industry scale from the brains directly. In this work, a simple and highly efficient method with high yield was developed for the isolation, conversion, and purification of GM1 from a pig brain. Gangliosides (GLS) were first extracted by supercritical CO₂ (SCE). The optimum extraction time of GLS by SCE was 4 h, and the ratio of entrainer to acetone powder from the pig brain was 3:1 (<i>v/w</i>). GM1 was then prepared from GLS by immobilized sialidase and purified by reverse-phase silica gel. Sodium alginate embedding was used for the immobilization of sialidase. Under the optimized method, the yield of high-purity GM1 was around 0.056%. This method has the potential to be applied in the production of GM1 in the industry.