Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons

Abstract The spinal dorsal horn harbors a sophisticated and heterogeneous network of excitatory and inhibitory neurons that process peripheral signals encoding different sensory modalities. Although it has long been recognized that this network is crucial both for the separation and the integration...

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Main Authors: Rebecca Rani Das Gupta, Louis Scheurer, Pawel Pelczar, Hendrik Wildner, Hanns Ulrich Zeilhofer
Format: Article
Language:English
Published: Nature Publishing Group 2021-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-84667-y
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spelling doaj-7ed2f83328d34b1d85c69a4326b330812021-03-11T12:19:42ZengNature Publishing GroupScientific Reports2045-23222021-03-0111111810.1038/s41598-021-84667-yNeuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neuronsRebecca Rani Das Gupta0Louis Scheurer1Pawel Pelczar2Hendrik Wildner3Hanns Ulrich Zeilhofer4Institute of Pharmacology and Toxicology, University of ZurichInstitute of Pharmacology and Toxicology, University of ZurichCenter for Transgenic Models, University of BaselInstitute of Pharmacology and Toxicology, University of ZurichInstitute of Pharmacology and Toxicology, University of ZurichAbstract The spinal dorsal horn harbors a sophisticated and heterogeneous network of excitatory and inhibitory neurons that process peripheral signals encoding different sensory modalities. Although it has long been recognized that this network is crucial both for the separation and the integration of sensory signals of different modalities, a systematic unbiased approach to the use of specific neuromodulatory systems is still missing. Here, we have used the translating ribosome affinity purification (TRAP) technique to map the translatomes of excitatory glutamatergic (vGluT2+) and inhibitory GABA and/or glycinergic (vGAT+ or Gad67+) neurons of the mouse spinal cord. Our analyses demonstrate that inhibitory and excitatory neurons are not only set apart, as expected, by the expression of genes related to the production, release or re-uptake of their principal neurotransmitters and by genes encoding for transcription factors, but also by a differential engagement of neuromodulator, especially neuropeptide, signaling pathways. Subsequent multiplex in situ hybridization revealed eleven neuropeptide genes that are strongly enriched in excitatory dorsal horn neurons and display largely non-overlapping expression patterns closely adhering to the laminar and presumably also functional organization of the spinal cord grey matter.https://doi.org/10.1038/s41598-021-84667-y
collection DOAJ
language English
format Article
sources DOAJ
author Rebecca Rani Das Gupta
Louis Scheurer
Pawel Pelczar
Hendrik Wildner
Hanns Ulrich Zeilhofer
spellingShingle Rebecca Rani Das Gupta
Louis Scheurer
Pawel Pelczar
Hendrik Wildner
Hanns Ulrich Zeilhofer
Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
Scientific Reports
author_facet Rebecca Rani Das Gupta
Louis Scheurer
Pawel Pelczar
Hendrik Wildner
Hanns Ulrich Zeilhofer
author_sort Rebecca Rani Das Gupta
title Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_short Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_full Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_fullStr Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_full_unstemmed Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
title_sort neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-03-01
description Abstract The spinal dorsal horn harbors a sophisticated and heterogeneous network of excitatory and inhibitory neurons that process peripheral signals encoding different sensory modalities. Although it has long been recognized that this network is crucial both for the separation and the integration of sensory signals of different modalities, a systematic unbiased approach to the use of specific neuromodulatory systems is still missing. Here, we have used the translating ribosome affinity purification (TRAP) technique to map the translatomes of excitatory glutamatergic (vGluT2+) and inhibitory GABA and/or glycinergic (vGAT+ or Gad67+) neurons of the mouse spinal cord. Our analyses demonstrate that inhibitory and excitatory neurons are not only set apart, as expected, by the expression of genes related to the production, release or re-uptake of their principal neurotransmitters and by genes encoding for transcription factors, but also by a differential engagement of neuromodulator, especially neuropeptide, signaling pathways. Subsequent multiplex in situ hybridization revealed eleven neuropeptide genes that are strongly enriched in excitatory dorsal horn neurons and display largely non-overlapping expression patterns closely adhering to the laminar and presumably also functional organization of the spinal cord grey matter.
url https://doi.org/10.1038/s41598-021-84667-y
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