Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.
Main Authors: | Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-01-01
|
Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/ncomms14261 |
Similar Items
-
Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
by: Yi Zhang, et al.
Published: (2016-08-01) -
Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
by: Sergei Svitashev, et al.
Published: (2016-11-01) -
CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells
by: Andrew D. Hildreth, et al.
Published: (2020-12-01) -
Targeted Gene Editing in Human Primary T Cells Using CRISPR/Cas9 Ribonucleoproteins
by: Elahe Kamali, et al.
Published: (2021-04-01) -
Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana
by: Shaoping Wu, et al.
Published: (2020-09-01)