Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.

Bibliographic Details
Main Authors:	Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-01-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms14261

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spelling	doaj-7eca31ccd8a84cdba06a1486c2d465922021-05-11T07:39:50ZengNature Publishing GroupNature Communications2041-17232017-01-01811510.1038/ncomms14261Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexesZhen Liang0Kunling Chen1Tingdong Li2Yi Zhang3Yanpeng Wang4Qian Zhao5Jinxing Liu6Huawei Zhang7Cuimin Liu8Yidong Ran9Caixia Gao10State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesGenovo Biotechnology Co., LtdState Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of SciencesProtocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.https://doi.org/10.1038/ncomms14261
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Zhen Liang Kunling Chen Tingdong Li Yi Zhang Yanpeng Wang Qian Zhao Jinxing Liu Huawei Zhang Cuimin Liu Yidong Ran Caixia Gao
spellingShingle	Zhen Liang Kunling Chen Tingdong Li Yi Zhang Yanpeng Wang Qian Zhao Jinxing Liu Huawei Zhang Cuimin Liu Yidong Ran Caixia Gao Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes Nature Communications
author_facet	Zhen Liang Kunling Chen Tingdong Li Yi Zhang Yanpeng Wang Qian Zhao Jinxing Liu Huawei Zhang Cuimin Liu Yidong Ran Caixia Gao
author_sort	Zhen Liang
title	Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_short	Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_full	Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_fullStr	Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_full_unstemmed	Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
title_sort	efficient dna-free genome editing of bread wheat using crispr/cas9 ribonucleoprotein complexes
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2017-01-01
description	Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al. show that CRISPR/Cas9 ribonucleoproteins can be used to generate genome edited wheat plants in as little as nine weeks.
url	https://doi.org/10.1038/ncomms14261
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Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

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