Isolation of diuron-degrading bacteria from treated soil
Studies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period....
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2004-11-01
|
Series: | Brazilian Archives of Biology and Technology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020 |
id |
doaj-7ebb91ccd2514e348e8a11b8cf2aedcc |
---|---|
record_format |
Article |
spelling |
doaj-7ebb91ccd2514e348e8a11b8cf2aedcc2020-11-24T20:47:15ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242004-11-01476999100310.1590/S1516-89132004000600020Isolation of diuron-degrading bacteria from treated soilPriscila Maria DellamatriceRegina Teresa Rosim MonteiroStudies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period. In the first soil there was a significant increase in the number of bacteria, from 3.3 x 10 6 to 1.9 x 10(8). The microbial biomass did not change, however, a significant 14C-residue of diuron was found in the microbial biomass. A consortium of three bacteria, Acinetobacter johnsonii and two Bacillus spp., was isolated in medium containing diuron as the only carbon source. Only A. johnsonii was able to grow alone in medium with diuron as the only carbon source.<br>A degradação do herbicida diuron foi estudada em solo com e sem histórico de aplicação obtendo-se aproximadamente degradação sete vezes maior no solo com histórico de aplicação, após 64 dias de incubação. Houve um aumento no número de bactérias no solo com histórico de aplicação de 3.3 x 10 6 para 1.9 x 10(8) UFC. g-1 solo. Não houve aumento na biomassa após a incubação, porém foi encontrado significante resíduo de 14C-diuron na biomassa. Um consórcio de três bactérias foi isolado, Acinetobacter johnsonii e duas espécies de Bacillus sp., em meio contendo diuron como única fonte de carbono. Somente A. johnsonnii foi capaz de crescer sozinha em meio contendo diuron como única fonte de carbono.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020Biodegradationbiomassenhanced degradationdiuronAcinetobacterbioremediation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Priscila Maria Dellamatrice Regina Teresa Rosim Monteiro |
spellingShingle |
Priscila Maria Dellamatrice Regina Teresa Rosim Monteiro Isolation of diuron-degrading bacteria from treated soil Brazilian Archives of Biology and Technology Biodegradation biomass enhanced degradation diuron Acinetobacter bioremediation |
author_facet |
Priscila Maria Dellamatrice Regina Teresa Rosim Monteiro |
author_sort |
Priscila Maria Dellamatrice |
title |
Isolation of diuron-degrading bacteria from treated soil |
title_short |
Isolation of diuron-degrading bacteria from treated soil |
title_full |
Isolation of diuron-degrading bacteria from treated soil |
title_fullStr |
Isolation of diuron-degrading bacteria from treated soil |
title_full_unstemmed |
Isolation of diuron-degrading bacteria from treated soil |
title_sort |
isolation of diuron-degrading bacteria from treated soil |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1516-8913 1678-4324 |
publishDate |
2004-11-01 |
description |
Studies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period. In the first soil there was a significant increase in the number of bacteria, from 3.3 x 10 6 to 1.9 x 10(8). The microbial biomass did not change, however, a significant 14C-residue of diuron was found in the microbial biomass. A consortium of three bacteria, Acinetobacter johnsonii and two Bacillus spp., was isolated in medium containing diuron as the only carbon source. Only A. johnsonii was able to grow alone in medium with diuron as the only carbon source.<br>A degradação do herbicida diuron foi estudada em solo com e sem histórico de aplicação obtendo-se aproximadamente degradação sete vezes maior no solo com histórico de aplicação, após 64 dias de incubação. Houve um aumento no número de bactérias no solo com histórico de aplicação de 3.3 x 10 6 para 1.9 x 10(8) UFC. g-1 solo. Não houve aumento na biomassa após a incubação, porém foi encontrado significante resíduo de 14C-diuron na biomassa. Um consórcio de três bactérias foi isolado, Acinetobacter johnsonii e duas espécies de Bacillus sp., em meio contendo diuron como única fonte de carbono. Somente A. johnsonnii foi capaz de crescer sozinha em meio contendo diuron como única fonte de carbono. |
topic |
Biodegradation biomass enhanced degradation diuron Acinetobacter bioremediation |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020 |
work_keys_str_mv |
AT priscilamariadellamatrice isolationofdiurondegradingbacteriafromtreatedsoil AT reginateresarosimmonteiro isolationofdiurondegradingbacteriafromtreatedsoil |
_version_ |
1716810546312904704 |