Isolation of diuron-degrading bacteria from treated soil

Studies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period....

Full description

Bibliographic Details
Main Authors: Priscila Maria Dellamatrice, Regina Teresa Rosim Monteiro
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2004-11-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020
id doaj-7ebb91ccd2514e348e8a11b8cf2aedcc
record_format Article
spelling doaj-7ebb91ccd2514e348e8a11b8cf2aedcc2020-11-24T20:47:15ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242004-11-01476999100310.1590/S1516-89132004000600020Isolation of diuron-degrading bacteria from treated soilPriscila Maria DellamatriceRegina Teresa Rosim MonteiroStudies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period. In the first soil there was a significant increase in the number of bacteria, from 3.3 x 10 6 to 1.9 x 10(8). The microbial biomass did not change, however, a significant 14C-residue of diuron was found in the microbial biomass. A consortium of three bacteria, Acinetobacter johnsonii and two Bacillus spp., was isolated in medium containing diuron as the only carbon source. Only A. johnsonii was able to grow alone in medium with diuron as the only carbon source.<br>A degradação do herbicida diuron foi estudada em solo com e sem histórico de aplicação obtendo-se aproximadamente degradação sete vezes maior no solo com histórico de aplicação, após 64 dias de incubação. Houve um aumento no número de bactérias no solo com histórico de aplicação de 3.3 x 10 6 para 1.9 x 10(8) UFC. g-1 solo. Não houve aumento na biomassa após a incubação, porém foi encontrado significante resíduo de 14C-diuron na biomassa. Um consórcio de três bactérias foi isolado, Acinetobacter johnsonii e duas espécies de Bacillus sp., em meio contendo diuron como única fonte de carbono. Somente A. johnsonnii foi capaz de crescer sozinha em meio contendo diuron como única fonte de carbono.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020Biodegradationbiomassenhanced degradationdiuronAcinetobacterbioremediation
collection DOAJ
language English
format Article
sources DOAJ
author Priscila Maria Dellamatrice
Regina Teresa Rosim Monteiro
spellingShingle Priscila Maria Dellamatrice
Regina Teresa Rosim Monteiro
Isolation of diuron-degrading bacteria from treated soil
Brazilian Archives of Biology and Technology
Biodegradation
biomass
enhanced degradation
diuron
Acinetobacter
bioremediation
author_facet Priscila Maria Dellamatrice
Regina Teresa Rosim Monteiro
author_sort Priscila Maria Dellamatrice
title Isolation of diuron-degrading bacteria from treated soil
title_short Isolation of diuron-degrading bacteria from treated soil
title_full Isolation of diuron-degrading bacteria from treated soil
title_fullStr Isolation of diuron-degrading bacteria from treated soil
title_full_unstemmed Isolation of diuron-degrading bacteria from treated soil
title_sort isolation of diuron-degrading bacteria from treated soil
publisher Instituto de Tecnologia do Paraná (Tecpar)
series Brazilian Archives of Biology and Technology
issn 1516-8913
1678-4324
publishDate 2004-11-01
description Studies were carried out on the isolation of diuron-degrading bacteria from treated soil. The mineralization of 14C-diuron in soil following a three-year application and in soil without previous application were 68.95 mug.100 g-1 and 24.16 mug.100 g-1, respectively, after a 64-day incubation period. In the first soil there was a significant increase in the number of bacteria, from 3.3 x 10 6 to 1.9 x 10(8). The microbial biomass did not change, however, a significant 14C-residue of diuron was found in the microbial biomass. A consortium of three bacteria, Acinetobacter johnsonii and two Bacillus spp., was isolated in medium containing diuron as the only carbon source. Only A. johnsonii was able to grow alone in medium with diuron as the only carbon source.<br>A degradação do herbicida diuron foi estudada em solo com e sem histórico de aplicação obtendo-se aproximadamente degradação sete vezes maior no solo com histórico de aplicação, após 64 dias de incubação. Houve um aumento no número de bactérias no solo com histórico de aplicação de 3.3 x 10 6 para 1.9 x 10(8) UFC. g-1 solo. Não houve aumento na biomassa após a incubação, porém foi encontrado significante resíduo de 14C-diuron na biomassa. Um consórcio de três bactérias foi isolado, Acinetobacter johnsonii e duas espécies de Bacillus sp., em meio contendo diuron como única fonte de carbono. Somente A. johnsonnii foi capaz de crescer sozinha em meio contendo diuron como única fonte de carbono.
topic Biodegradation
biomass
enhanced degradation
diuron
Acinetobacter
bioremediation
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600020
work_keys_str_mv AT priscilamariadellamatrice isolationofdiurondegradingbacteriafromtreatedsoil
AT reginateresarosimmonteiro isolationofdiurondegradingbacteriafromtreatedsoil
_version_ 1716810546312904704